RGD Reference Report - Inhibitory effect of recombinant IL-25 on the development of dextran sulfate sodium-induced experimental colitis in mice. - Rat Genome Database

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Inhibitory effect of recombinant IL-25 on the development of dextran sulfate sodium-induced experimental colitis in mice.

Authors: Mchenga, S S Salum  Wang, Danan  Li, Cheng  Shan, Fengping  Lu, Changlong 
Citation: Mchenga SS, etal., Cell Mol Immunol. 2008 Dec;5(6):425-31. doi: 10.1038/cmi.2008.53.
RGD ID: 39456129
Pubmed: PMID:19118508   (View Abstract at PubMed)
PMCID: PMC4072414   (View Article at PubMed Central)
DOI: DOI:10.1038/cmi.2008.53   (Journal Full-text)

The role of interleukin 25 (IL-25) in a number of human diseases still has not been extensively studied, here we attempt to evaluate the role of recombinant IL-25 (rIL-25) in the development of dextran sulfate sodium (DSS)-induced experimental colitis. Acute colitis was induced in female C57BL/6 mice by oral administration of 2.5% DSS in drinking water ad libitum. At the same time as the start of DSS exposure, mice were injected intraperitoneally with 0.4 microg of rIL-25 or PBS. Then disease activity index (DAI), histological changes and survival rate were observed. The levels of IL-17, IL-23, and TGF-beta1 in colon tissues were determined by ELISA, and the production of IL-17 by CD4(+)/CD8(+) T cells was detected by intracellular flow cytometry. In contrast to the DSS treated mice, DSS + rIL-25 treated mice displayed a lower DAI, limited histological changes and prolonged survival. The levels of IL-23 and TGF-beta1 were significantly elevated in the DSS + rIL-25 treated mice compared to the DSS treated mice. There was no significant difference in the production of IL-17 in colon tissues and CD4(+)/CD8(+) T cells between the DSS + rIL-25 treated mice and DSS treated mice. Our findings suggest the role of IL-25 in inhibiting development and progression of acute colitis in DSS-induced mouse colitis model.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Experimental Colitis treatmentISOIl25 (Mus musculus)39456129; 39456129 RGD 
Experimental Colitis treatmentIDA 39456129 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Il25  (interleukin 25)

Genes (Mus musculus)
Il25  (interleukin 25)

Genes (Homo sapiens)
IL25  (interleukin 25)


Additional Information