RGD Reference Report - Infertility associated with meiotic failure in the tremor rat (tm/tm) is caused by the deletion of spermatogenesis associated 22. - Rat Genome Database

RGD Reference Report - Infertility associated with meiotic failure in the tremor rat (tm/tm) is caused by the deletion of spermatogenesis associated 22. - Rat Genome Database

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General

Infertility associated with meiotic failure in the tremor rat (tm/tm) is caused by the deletion of spermatogenesis associated 22.
Authors:	Ishishita, Satoshi Inui, Toshihide Matsuda, Yoichi Serikawa, Tadao Kitada, Kazuhiro
Citation:	Ishishita S, etal., Exp Anim. 2013;62(3):219-27. doi: 10.1538/expanim.62.219.
RGD ID:	38549346
Pubmed:	PMID:23903057 (View Abstract at PubMed)
PMCID:	PMC4160939 (View Article at PubMed Central)
DOI:	DOI:10.1538/expanim.62.219 (Journal Full-text)
The tremor rat is an autosomal recessive mutant exhibiting sterility with gonadal hypoplasia in both sexes. The causative mutation tremor (tm) is known as a genomic deletion spanning >200 kb in Chr 10q24. Spermatogenesis associated 22 (Spata22) has been shown to be a vertebrate-specific gene essential for the progression of meiosis through prophase I and completion of chromosome synapsis and meiotic recombination using a mouse repro42 mutant carrying an N-ethyl-N-nitrosourea (ENU)-induced nonsense mutation in Spata22. In this study, we show that Spata22 was identified as the gene responsible for the failure of gametogenesis to progress beyond meiosis I in tm homozygous rats by a transgenic rescue experiment. Meiosis was arrested during prophase I in the mutant testis. Precise mapping of the breakage point revealed that the deleted genomic region spanned approximately 240 kb and comprised at least 13 genes, including Spata22. Rat Spata22 was predominantly expressed in the testis, and its transcription increased with the first wave of spermatogenesis, as seen in the mouse ortholog. These results suggest that Spata22 may play an important role in meiotic prophase I in rats, as seen in mice, and that the tm homozygous rat may be useful for investigating the physiological function of Spata22, as an experimental system for clarifying the effect of a null mutation, and may be an animal model for studying the pathogenesis and treatment of infertility caused by impaired meiosis.

Gene Ontology Annotations Click to see Annotation Detail View

Biological Process

Only show annotations with direct experimental evidence (0 objects hidden)

Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
spermatocyte division		IMP		38549346		RGD

Phenotype Annotations Click to see Annotation Detail View

Mammalian Phenotype

Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
abnormal meiosis	treatment	IDA		38549346		RGD
abnormal meiosis	treatment	IDA	gene transfer using plasmid vector	38549346	compared to untreated	RGD
arrest of spermiogenesis	treatment	IDA		38549346		RGD
arrest of spermiogenesis		IDA	gene transfer using plasmid vector	38549346	compared to untreated	RGD
decreased testis weight	treatment	IDA		38549346		RGD
decreased testis weight	treatment	IDA	gene transfer using plasmid vector	38549346	compared to untreated	RGD
ovary degeneration	treatment	IDA		38549346		RGD
ovary degeneration	treatment	IDA	gene transfer using plasmid vector	38549346	compared to untreated	RGD

Objects Annotated

Genes (Rattus norvegicus)

Spata22 (spermatogenesis associated 22)

Strains

TRM/Kyo (tremor rat)

Additional Information