RGD Reference Report - Lung epithelium is the major source of IL-33 and is regulated by IL-33-dependent and IL-33-independent mechanisms in pulmonary cryptococcosis. - Rat Genome Database

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Lung epithelium is the major source of IL-33 and is regulated by IL-33-dependent and IL-33-independent mechanisms in pulmonary cryptococcosis.

Authors: Heyen, Laura  Müller, Uwe  Siegemund, Sabine  Schulze, Bianca  Protschka, Martina  Alber, Gottfried  Piehler, Daniel 
Citation: Heyen L, etal., Pathog Dis. 2016 Oct;74(7). pii: ftw086. doi: 10.1093/femspd/ftw086. Epub 2016 Sep 4.
RGD ID: 38549345
Pubmed: (View Article at PubMed) PMID:27596810
DOI: Full-text: DOI:10.1093/femspd/ftw086

Inhalation of the fungus Cryptococcus neoformans (C. neoformans) results in pulmonary cryptococcosis associated with IL-33-dependent type 2 immunity. Lung epithelium represents the initial contact site of infection. The role of IL-33 in type 2 immunity has been analyzed, but the source of this cytokine and its effect on lung epithelial cell function in pulmonary cryptococcosis remained unclear. In mice infected with C. neoformans, we identified alveolar type 2 epithelial cells as major source of IL-33. On both, IL-33-positive and IL-33-negative epithelial cells, IL-33 receptor expression was detectable. Therefore, we studied the role of IL-33 receptor expression for IL-33 synthesis during fungal infection on lung epithelial cells and found no auto-/paracrine IL-33 induction. Next, the effect of IL-33 on epithelial E-cadherin expression, a cell-to-cell adhesion molecule, was analyzed. Fungal infection resulted in E-cadherin downregulation in an IL-33-dependent manner on pulmonary epithelial cells both at the single-cell and at the population level. On the other hand, epithelial cells from infected mice upregulated surfactant protein C (SP-C) and CXCL15 mRNA production together with but independently of IL-33. In conclusion, lung epithelium represents a significant source of IL-33 in pulmonary cryptococcosis and is regulated in an IL-33-dependent but also IL-33-independent manner.



Disease Annotations    
cryptococcosis  (IEP,ISO)

Objects Annotated

Genes (Rattus norvegicus)
Il33  (interleukin 33)
Sftpc  (surfactant protein C)

Genes (Mus musculus)
Cxcl15  (chemokine (C-X-C motif) ligand 15)
Il33  (interleukin 33)
Sftpc  (surfactant associated protein C)

Genes (Homo sapiens)
CXCL8  (C-X-C motif chemokine ligand 8)
IL33  (interleukin 33)
SFTPC  (surfactant protein C)


Additional Information