RGD Reference Report - Interplay between collagenase and undescended testes in Adamts16 knockout rats. - Rat Genome Database

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Interplay between collagenase and undescended testes in Adamts16 knockout rats.

Authors: Sarila, Gulcan  Bao, Tuya  Abeydeera, Sanduni Amaya  Li, Ruili  Mell, Blair  Joe, Bina  Catubig, Angelique  Hutson, John 
Citation: Sarila G, etal., J Pediatr Surg. 2020 Jan 7. pii: S0022-3468(19)30929-7. doi: 10.1016/j.jpedsurg.2019.12.019.
RGD ID: 38548917
Pubmed: PMID:32037220   (View Abstract at PubMed)
DOI: DOI:10.1016/j.jpedsurg.2019.12.019   (Journal Full-text)


BACKGROUND: The inguinoscrotal stage of testicular descent is characterized by an increase in cell density and collagen fibers as the gubernaculum undergoes cell division and increases Extracellular Matrix (ECM) activity. Rats that lack the enzyme Adamts16, a known ECM proteinase, develop cryptorchidism postnatally and are infertile. Therefore, this study aims to investigate the link between the Adamts16 enzyme and congenital undescended testes (UDT) in Adamts16 knockout (KO) rats during postnatal development.
METHODS: Formalin-fixed specimens of Wild-Type, Adamts16 heterozygous and Adamts16 homozygous KO rats post birth were sectioned and used for standard H&E histology and Masson's trichrome staining. A quantitative analysis on image J was performed to determine the intensity of collagen fibers within the inguinoscrotal fat pad (IFP) (n = 3 age/genotype).
RESULTS: The migration of the gubernaculum within the Adamts16 heterozygous and Adamts16 KO rat was considerably disrupted. Furthermore, the Masson's trichrome staining demonstrated a significant increase in collagen fibers around the gubernaculum of rats that lacked Adamts16 enzyme at day 8.
CONCLUSION: This study reports a failure of gubernacular migration leading to UDT in Adamts16 KO rats during development, suggesting that the expression of Adamts16 gene is critical for normal gubernacular migration through the breakdown of collagen fibers within the IFP.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cryptorchidism  ISOAdamts16 (Rattus norvegicus)38548917; 38548917 RGD 
cryptorchidism  IMP 38548917; 38548917 RGD 
cryptorchidism MODEL: spontaneousIMP 38548917 RGD 
male infertility  ISOAdamts16 (Rattus norvegicus)38548917; 38548917 RGD 
male infertility  IMP 38548917; 38548917 RGD 
male infertility MODEL: spontaneousIMP 38548917 RGD 

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
increased collagen level  IMP 38548917; 38548917; 38548917 RGD 
Objects Annotated

Genes (Rattus norvegicus)
Adamts16  (ADAM metallopeptidase with thrombospondin type 1 motif, 16)
Adamts16em1Bj  (ADAM metallopeptidase with thrombospondin type 1 motif, 16; ZFN mutant1,Bj)

Genes (Mus musculus)
Adamts16  (ADAM metallopeptidase with thrombospondin type 1 motif 16)

Genes (Homo sapiens)
ADAMTS16  (ADAM metallopeptidase with thrombospondin type 1 motif 16)

Strains
SS-Adamts16em1Bj  (NA)


Additional Information