RGD Reference Report - Identification of SAA and ACTB as potential biomarker of patients with severe HFMD using iTRAQ quantitative proteomics. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Identification of SAA and ACTB as potential biomarker of patients with severe HFMD using iTRAQ quantitative proteomics.

Authors: Zhou, Fangye  Chen, Xianqian  Chen, Guoxian  Yan, Junhua  Xiao, Yupeng 
Citation: Zhou F, etal., Clin Biochem. 2019 May;67:1-6. doi: 10.1016/j.clinbiochem.2019.02.011. Epub 2019 Feb 25.
RGD ID: 36947391
Pubmed: (View Article at PubMed) PMID:30817906
DOI: Full-text: DOI:10.1016/j.clinbiochem.2019.02.011

Hand, foot and mouth disease (HFMD) is an infectious disease caused by a variety of enterovirus infections, and the most common types of virus infections are the newenterovirus71 (EV71) and coxsackievirus A group 16 (CoxA16). A small fraction of HFMD will cause further severe HFMD. A rapid and accurate diagnosis biomarker of severe HFMD is important for the timely treatment. In the study, we conducted a clinical biomarker discovery study using iTRAQ combined with MS. Serum proteome alterations in severe HFMD group (n = 32) and health control group (n = 32) were analyzed. 47 proteins were upregulated (fold change > 1.5) between the severe HFMD group and HC group. The identified proteins were classified into different groups according to the molecular function, biology processes, cellular component. During the up-regulated proteins, serum amyloid A (SAA) and human β-actin (ACTB), were confirmed in the serum of the severe HFMD and HC by ELISA assay. SAA and ACTB levels were significantly higher in the sever HFMD patients (P < .01), consistent with iTRAQ-LC-MS/MS analysis. In summary, Our results showed that SAA and human β-actin (ACTB) may be served as a potential biomarker of the clinical diagnosis of severe HFMD.

Annotation

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Actb  (actin, beta)

Genes (Mus musculus)
Actb  (actin, beta)

Genes (Homo sapiens)
ACTB  (actin beta)


Additional Information