RGD Reference Report - Cystic fibrosis transmembrane conductance regulator in the endolymphatic sac of the rat. - Rat Genome Database

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Cystic fibrosis transmembrane conductance regulator in the endolymphatic sac of the rat.

Authors: Matsubara, Ai  Miyashita, Takenori  Inamoto, Ryuhei  Hoshikawa, Hiroshi  Mori, Nozomu 
Citation: Matsubara A, etal., Auris Nasus Larynx. 2014 Oct;41(5):409-12. doi: 10.1016/j.anl.2014.02.005. Epub 2014 Mar 2.
RGD ID: 35673329
Pubmed: PMID:24598307   (View Abstract at PubMed)
DOI: DOI:10.1016/j.anl.2014.02.005   (Journal Full-text)


OBJECTIVE: Na(+) and Cl(-) are dominant ions in the endolymphatic fluid in the endolymphatic sac and are important for volume regulation in the endolymphatic sac. An epithelial sodium channel (ENaC) and other Na(+) transporters have been identified in the endolymphatic sac epithelia, and they are involved in the regulation of endolymph. Although the presence of Cl(-) channels in the endolymphatic sac epithelia has been speculated, no Cl(-) channels have been identified. In this study, we confirmed the expression of cystic fibrosis transmembrane conductance regulator (CFTR) in the endolymphatic sac by reverse transcriptase polymerase chain reaction (RT-PCR) and by immunohistochemical staining.
METHODS: Pure mRNA from endolymphatic sac epithelia was prepared using laser capture microdissection (LCM) and examined using RT-PCR. Localization of CFTR and ENaC in the endolymphatic sac was examined using immunohistochemistry.
RESULTS: mRNA of the CFTR was expressed in the endolymphatic sac. Immunohistochemical analysis showed the expression of the CFTR on apical side of the endolymphatic sac epithelia and co-localization with the ENaC.
CONCLUSION: RT-PCR and immunohistochemistry were used to identify the expression of CFTR in the endolymphatic sac epithelia, which gives us a clue for understanding Cl(-) transport in the endolymphatic sac. These results suggest a pathway for Cl(-), possibly through interaction with the ENaC, which may regulate the endolymph in the endolymphatic sac.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
apical plasma membrane  IDA 35673329 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cftr  (CF transmembrane conductance regulator)


Additional Information