RGD Reference Report - Role of HMGB1 in Post-traumatic Endoplasmic Reticulum Stress in Rat Lung Tissues. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Role of HMGB1 in Post-traumatic Endoplasmic Reticulum Stress in Rat Lung Tissues.

Authors: Lu, J F  Zhang, Q J  Li, X H  Liu, G Q  Liu, Y C  Gu, Z Y 
Citation: Lu JF, etal., Fa Yi Xue Za Zhi. 2018 Aug;34(4):347-351. doi: 10.12116/j.issn.1004-5619.2018.04.001. Epub 2018 Aug 25.
RGD ID: 34901874
Pubmed: PMID:30465396   (View Abstract at PubMed)
DOI: DOI:10.12116/j.issn.1004-5619.2018.04.001   (Journal Full-text)


OBJECTIVES: To explore the role of high mobility group B1 (HMGB1) protein in the post-traumatic endoplasmic reticulum stress (ERS) in rat lung tissues.
METHODS: The rat model of acute lung injury was established by crushing the hind limbs of rats with standard weight. The first experiment was to divide rats into postural control group and crush groups (6 h, 18 h and 30 h after crushing). The second experiment was to divide rats into postural control group, 18 h crush group, HMGB1 inhibitor sodium butyrate (SB) group and 18 h crush+SB group. The protein expression changes of HMGB1 and ERS- related proteins (GRP78, caspase-12, CHOP and IRE1α) in rat lung tissues were detected with Western blotting. Meanwhile, the pathological changes of rat lungs were observed by HE stain.
RESULTS: Compared with the postural control group, the expression levels of ERS-related proteins (GRP78, caspase-12, CHOP and IRE1α) and HMGB1 protein in rat lung tissues by crushing the hind limbs of rats were obviously increased. The protein levels reduced at 30 h after crushing but were still higher than those of postural control group and obvious pathological changes of acute lung injury were observed simultaneously in rats. Compared with the 18 h crush group, the expression levels of the ERS-related proteins and HMGB1 protein in rat lung tissues were attenuated in 18 h crush+SB group, and the pathological changes of rat lung injury began to alleviate.
CONCLUSIONS: HMGB1-ERS pathway activated by traumatic stress can lead to acute lung injury in rats.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Acute Lung Injury treatmentISOCasp12 (Rattus norvegicus)34901874; 34901874associated with Crush InjuriesRGD 
Acute Lung Injury treatmentIEP 34901874; 34901874; 34901874; 34901874; 34901874associated with Crush InjuriesRGD 
Acute Lung Injury treatmentISODdit3 (Rattus norvegicus)34901874; 34901874associated with Crush InjuriesRGD 
Acute Lung Injury treatmentISOErn1 (Rattus norvegicus)34901874; 34901874associated with Crush InjuriesRGD 
Acute Lung Injury treatmentISOHmgb1 (Rattus norvegicus)34901874; 34901874associated with Crush InjuriesRGD 
Acute Lung Injury treatmentISOHspa5 (Rattus norvegicus)34901874; 34901874associated with Crush InjuriesRGD 

Objects Annotated

Genes (Rattus norvegicus)
Casp12  (caspase 12)
Ddit3  (DNA-damage inducible transcript 3)
Ern1  (endoplasmic reticulum to nucleus signaling 1)
Hmgb1  (high mobility group box 1)
Hspa5  (heat shock protein family A (Hsp70) member 5)

Genes (Mus musculus)
Casp12  (caspase 12)
Ddit3  (DNA-damage inducible transcript 3)
Ern1  (endoplasmic reticulum to nucleus signalling 1)
Hmgb1  (high mobility group box 1)
Hspa5  (heat shock protein 5)

Genes (Homo sapiens)
CASP12  (caspase 12 (gene/pseudogene))
DDIT3  (DNA damage inducible transcript 3)
ERN1  (endoplasmic reticulum to nucleus signaling 1)
HMGB1  (high mobility group box 1)
HSPA5  (heat shock protein family A (Hsp70) member 5)


Additional Information