RGD Reference Report - Ischemic limb preconditioning downregulates systemic inflammatory activation. - Rat Genome Database

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Ischemic limb preconditioning downregulates systemic inflammatory activation.

Authors: Szabó, Andrea  Varga, Renáta  Keresztes, Margit  Vízler, Csaba  Németh, István  Rázga, Zsolt  Boros, Mihály 
Citation: Szabó A, etal., J Orthop Res. 2009 Jul;27(7):897-902. doi: 10.1002/jor.20829.
RGD ID: 329902055
Pubmed: PMID:19105227   (View Abstract at PubMed)
DOI: DOI:10.1002/jor.20829   (Journal Full-text)

We examined local and systemic antiinflammatory consequences of ischemic preconditioning (IPC) in a rat model of limb ischemia-reperfusion (I-R) by characterizing the leukocyte-endothelial interactions in the periosteum and the expression of adhesion molecules playing a role in leukocyte-mediated inflammatory processes. IPC induction (2 cycles of 10 min of complete limb ischemia and 10 min of reperfusion) was followed by 60 min of ischemia/180 min of reperfusion or sham-operation. Data were compared with those on animals subjected to I-R and sham-operation. Neutrophil leukocyte-endothelial cell interactions (intravital videomicroscopy), intravascular neutrophil activation (CD11b expression changes by flow cytometry), and soluble and tissue intercellular adhesion molecule-1 (ICAM-1; ELISA and immunohistochemistry, respectively) expressions were assessed. I-R induced enhanced leukocyte rolling and adherence in the periosteal postcapillary venules after 120 and 180 min of reperfusion. This was associated with a significantly enhanced CD11b expression (by approximately 80% and 72%, respectively) and moderately increased soluble and periosteal ICAM-1 expressions. IPC prevented the I-R-induced increases in leukocyte adherence and CD11b expression without influencing the soluble and tissue ICAM-1 levels. The results show that limb IPC exerts not only local, but distant antiinflammatory effects through significant modulation of neutrophil recruitment.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
ITGAMHumanSkeletal Muscle Reperfusion Injury treatmentISOItgam (Rattus norvegicus) RGD 
ItgamRatSkeletal Muscle Reperfusion Injury treatmentIEP  RGD 
ItgamMouseSkeletal Muscle Reperfusion Injury treatmentISOItgam (Rattus norvegicus) RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
ItgamRatresponse to ischemia  IEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Itgam  (integrin subunit alpha M)

Genes (Mus musculus)
Itgam  (integrin alpha M)

Genes (Homo sapiens)
ITGAM  (integrin subunit alpha M)


Additional Information