RGD Reference Report - Extracellular matrix protein CCN1 regulates cardiomyocyte apoptosis in mice with stress-induced cardiac injury. - Rat Genome Database

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Extracellular matrix protein CCN1 regulates cardiomyocyte apoptosis in mice with stress-induced cardiac injury.

Authors: Hsu, Pei-Ling  Su, Bor-Chyuan  Kuok, Qian-Yu  Mo, Fan-E 
Citation: Hsu PL, etal., Cardiovasc Res. 2013 Apr 1;98(1):64-72. doi: 10.1093/cvr/cvt001. Epub 2013 Jan 16.
RGD ID: 329845572
Pubmed: PMID:23329650   (View Abstract at PubMed)
DOI: DOI:10.1093/cvr/cvt001   (Journal Full-text)


AIMS: Expression of extracellular matrix protein CCN1 is induced in end-stage ischaemic cardiomyopathy in humans, and after cardiac ischaemia and reperfusion in experimental animal models. Despite its well-documented angiogenic activities, CCN1 increases the cytotoxicities of the tumour necrosis factor family cytokines, which promotes apoptosis in fibroblasts. We aimed to determine the physiological function of CCN1 in an injured heart.
METHODS AND RESULTS: To assess the function of CCN1 in vivo, knock-in mice carrying the apoptosis-defective mutant allele Ccn1-dm were tested in an isoproterenol (ISO)-induced myocardial injury model (100 mg/kg/day of sc injected ISO for 5 days). Compared with wild-type mice, Ccn1(dm/dm) mice were remarkably resistant to ISO-induced cardiac injury; they showed no post-treatment cardiomyocyte apoptosis or myocardial tissue damage. ISO cardiotoxicity was dependent on Fas ligand (FasL) and its downstream signalling. Using primary cultures of cardiomyocytes isolated from rats, we demonstrated that CCN1 sensitized FasL-mediated apoptosis by engaging its cell-surface receptor integrin α6β1 and up-regulating intracellular reactive oxygen species (ROS), which activated mitogen-activated protein kinase p38, and increased cell-surface Fas expression.
CONCLUSION: CCN1 is a critical pathophysiological regulator that mediates cardiomyocyte apoptosis during work-overload-induced cardiac injury. CCN1 increases cellular susceptibility to Fas-induced apoptosis by increasing ROS and cell-surface Fas expression.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CCN1HumanAcute Heart Injury amelioratesISOCcn1 (Mus musculus) RGD 
Ccn1RatAcute Heart Injury amelioratesISOCcn1 (Mus musculus) RGD 
Ccn1MouseAcute Heart Injury amelioratesIMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ccn1  (cellular communication network factor 1)

Genes (Mus musculus)
Ccn1  (cellular communication network factor 1)

Genes (Homo sapiens)
CCN1  (cellular communication network factor 1)


Additional Information