RGD Reference Report - Glycine transporter isoforms show differential subcellular localization in PC12 cells. - Rat Genome Database

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Glycine transporter isoforms show differential subcellular localization in PC12 cells.

Authors: Geerlings, Arjan  Núñez, Enrique  Rodenstein, Lara  López-Corcuera, Beatriz  Aragón, Carmen 
Citation: Geerlings A, etal., J Neurochem. 2002 Jul;82(1):58-65. doi: 10.1046/j.1471-4159.2002.00930.x.
RGD ID: 30309946
Pubmed: PMID:12091465   (View Abstract at PubMed)
DOI: DOI:10.1046/j.1471-4159.2002.00930.x   (Journal Full-text)

The subcellular localization of glycine transporters one (GLYT1) and two (GLYT2) stably expressed in PC12 cells has been studied. To facilitate visualization, enhanced green fluorescent protein (GFP) was fused to the amino terminus of both glycine transporters. Functional analysis of the GFP-GLYT1 and GFP-GLYT2 stable cell lines demonstrated that they exhibited high affinity for glycine and the characteristic properties of both glycine transporter subtypes. The GFP-coupled transporters were differently distributed throughout the cell. GFP-GLYT1 was mainly localized on the plasma membrane, whereas most of GFP-GLYT2 was present on large dense-core vesicles and endosomes. Both transporters were absent from the synaptic vesicle population in PC12 cells.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc6a9Ratglycine import across plasma membrane involved_inIDA PMID:12091465ARUK-UCL 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc6a5Ratdense core granule located_inIDA PMID:12091465ARUK-UCL 
Slc6a9Ratdense core granule located_inIDA PMID:12091465ARUK-UCL 
Slc6a5Ratendosome located_inIDA PMID:12091465ARUK-UCL 
Slc6a9Ratendosome located_inIDA PMID:12091465ARUK-UCL 
Slc6a9Ratplasma membrane located_inIDA PMID:12091465ARUK-UCL 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc6a9Ratglycine transmembrane transporter activity enablesIDA PMID:12091465ARUK-UCL 

Objects Annotated

Genes (Rattus norvegicus)
Slc6a5  (solute carrier family 6 member 5)
Slc6a9  (solute carrier family 6 member 9)


Additional Information