RGD Reference Report - Carnitine precursor γ-butyrobetaine is a novel substrate of the Na(+)- and Cl(-)-dependent GABA transporter Gat2. - Rat Genome Database

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Carnitine precursor γ-butyrobetaine is a novel substrate of the Na(+)- and Cl(-)-dependent GABA transporter Gat2.

Authors: Nakanishi, Takeo  Fukuyama, Yoko  Fujita, Masaharu  Shirasaka, Yoshiyuki  Tamai, Ikumi 
Citation: Nakanishi T, etal., Drug Metab Pharmacokinet. 2011;26(6):632-6. doi: 10.2133/dmpk.dmpk-11-nt-053. Epub 2011 Oct 12.
RGD ID: 30309942
Pubmed: (View Article at PubMed) PMID:21997971
DOI: Full-text: DOI:10.2133/dmpk.dmpk-11-nt-053

To study transport of the carnitine precursor gamma-butyrobetaine (GBB) by rat liver-specific GABA transporter 2 (rGat2), we measured the uptake of deuterated GBB (d(3)-GBB) by Xenopus oocytes expressing rGat2. rGat2-mediated d(3)-GBB uptake was Na(+) and Cl(-) dependent, and was saturable with a K(m) value of 70.6 ± 34.8 µM. In carnitine transporter Octn2-deficient Jvs mice, mRNA expression of mGat3, the mouse ortholog of GAT2, decreased in liver, but increased in brain, while mRNA expression of mBbox1, which hydroxylates GBB to carnitine, increased in kidney and brain. These results indicate that GAT2 plays a role in transmembrane transport of GBB.



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Molecular Function

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Genes (Rattus norvegicus)
Slc6a13  (solute carrier family 6 member 13)


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