RGD Reference Report - HPV 16 E7 inhibits OSCC cell proliferation, invasion, and metastasis by upregulating the expression of miR-20a. - Rat Genome Database

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HPV 16 E7 inhibits OSCC cell proliferation, invasion, and metastasis by upregulating the expression of miR-20a.

Authors: Hu, Jun  Ge, Weili  Xu, Junfeng 
Citation: Hu J, etal., Tumour Biol. 2016 Jul;37(7):9433-40. doi: 10.1007/s13277-016-4817-4. Epub 2016 Jan 19.
RGD ID: 26884343
Pubmed: (View Article at PubMed) PMID:26781875
DOI: Full-text: DOI:10.1007/s13277-016-4817-4

The aim of this research was to study how HPV-16 E7 affects the proliferation, invasion, and metastasis of oral squamous cell carcinoma (OSCC) cells by upregulating the expression of miR-20a. A total of 60 OSCC patients were included in this study. SiRNA-198 was used to inhibit HPV-16 E7, and the constructed plasmid of HPV-16 E7 was transfected into Cal27 cells. Then, HPV-16 E7 protein was detected by Western blot and RT-PCR was performed to measure miR-20a expression in OSCC cells. Either HPV-16 E7 or the combination of HPV-16 E7 and miR-20a inhibitors was transfected into Cal27 cells separately. And then, the effect of miR-20a on OSCC cells proliferation was evaluated by CCK-8. Moreover, transwell assay and wound healing assay were used to assess the impact of miR-20a on OSCC cell invasion migration. MiR-20a was significantly higher in OSCC tissues compared with para-carcinoma tissues. RT-PCR results indicated that miR-20a was downregulated after silencing HPV-16 E7. By contrast, miR-20a was upregulated after the overexpression of HPV-16 E7. Upregulation of miR-20a by transfected plasmid HPV-16 E7 can significantly inhibit Cal27 cell proliferation, invasion, and migration. The expression of MiR-20a upregulated by HPV-16 E7 inhibits the proliferation, invasion, and migration of OSCC cells.

Annotation

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Mir20a  (microRNA 20a)

Genes (Mus musculus)
Mir20a  (microRNA 20a)

Genes (Homo sapiens)
MIR20A  (microRNA 20a)


Additional Information