RGD Reference Report - Cytokine and microRNA levels during different periods of paradoxical sleep deprivation and sleep recovery in rats. - Rat Genome Database

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Cytokine and microRNA levels during different periods of paradoxical sleep deprivation and sleep recovery in rats.

Authors: Brianza-Padilla, Malinalli  Sánchez-Muñoz, Fausto  Vázquez-Palacios, Gonzalo  Huang, Fengyang  Almanza-Pérez, Julio César  Bojalil, Rafael  Bonilla-Jaime, Herlinda 
Citation: Brianza-Padilla M, etal., PeerJ. 2018 Sep 13;6:e5567. doi: 10.7717/peerj.5567. eCollection 2018.
RGD ID: 25824951
Pubmed: PMID:30225174   (View Abstract at PubMed)
PMCID: PMC6139242   (View Article at PubMed Central)
DOI: DOI:10.7717/peerj.5567   (Journal Full-text)


Background: Sleep has a fundamental role in the regulation of homeostasis. The aim of this study was to assess the effect of different periods of paradoxical sleep deprivation (PSD) and recovery on serum levels of cytokines and miRNAs related to inflammatory responses.
Methods: Male Wistar rats were submitted to a PSD of 24, 96, or 192 h, or of 192 h followed by 20 days of recovery (192 h PSD+R). The concentrations of corticosterone, cytokines (IL-6, TNF, IL-10, Adiponectin) and miRNAs (miR-146a, miR-155, miR-223, miR-16, miR-126, miR-21) in serum were evaluated.
Results: At PSD 24 h a significant increase of IL-6 and decrease of IL-10 were observed. At PSD 96h adiponectin increased. At 192 h of PSD IL-6 increased significantly again, accompanied by a threefold increase of IL-10 and an increase of serum corticosterone. After 20 days of recovery (192 h PSD+R) corticosterone, IL-6 and TNF levels increased significantly, while IL-10 decreased also significantly. Regarding the miRNAs at 24 h of PSD serum miR-146a, miR-155, miR-223, and miR-16 levels all increased. At 96 h of PSD miR-223 decreased. At 192 h of PSD decreases in miR-16 and miR-126 were observed. After recovery serum miR-21 increased and miR-16 decreased.
Conclusion: PSD induces a dynamic response likely reflecting the induced cellular stress and manifested as variating hormonal and inflammatory responses. Sleep deprivation disturbed corticosterone, cytokine and miRNA levels in serum related to the duration of sleep deprivation, as short-term PSD produced effects similar to those of an acute inflammatory response and long-term PSD induced long-lasting disturbances of biological mediators.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Sleep Deprivation  ISOMir223 (Rattus norvegicus)25824951; 25824951 RGD 
Sleep Deprivation  IEP 25824951 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mir223  (microRNA 223)

Genes (Mus musculus)
Mir223  (microRNA 223)

Genes (Homo sapiens)
MIR223  (microRNA 223)


Additional Information