RGD Reference Report - Anti-tumor angiogenesis with a recombinant ag43/FGFR1 chimeric protein as a model antigen. - Rat Genome Database

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Anti-tumor angiogenesis with a recombinant ag43/FGFR1 chimeric protein as a model antigen.

Authors: Zheng, Shaoping  Weng, Zhihong  Zheng, Shaojiang  Guo, Junli  Huang, Fengying  Xie, Mingxing 
Citation: Zheng S, etal., J Huazhong Univ Sci Technolog Med Sci. 2010 Feb;30(1):25-8. doi: 10.1007/s11596-010-0105-5. Epub 2010 Feb 14.
RGD ID: 25440478
Pubmed: PMID:20155451   (View Abstract at PubMed)
DOI: DOI:10.1007/s11596-010-0105-5   (Journal Full-text)

In order to investigate the anti-tumor angiogenesis activity with a recombinant Ag43/FGFR1 chimeric protein (AF) vaccine in a mouse H22 hepatoma model, tumor volume and survival rate of the mice were studied at a 3-day interval. Microvessel density (MVD) was detected by immunohistochemistry. The endothelial deposition of autoantibodies within tumor tissues was examined by immunofluorescent staining, and anti-FGFR1 antibody-producing B cells (APBCs) were tested by enzyme-linked immunospot (ELISPOT) assay. Compared with the three control groups, the tumor volume was significantly decreased and the survival time was significantly prolonged in AF-immunized group (P<0.05). The number of APBCs in AF-immunized mice (129.6+/-10.9) was more than in controls [6.2+/-1.1 (FGFR1), 6.0+/-1.2 (Ag43) and 5.2+/-1.4 (NS), P<0.01]. Moreover, the endothelial deposition of autoantibodies was found in tumor tissues from AF-immunized mice, but not in control groups. MVD in AF-immunized group was significantly lower than in FGFR1-immunized group, Ag43-immunized group and NS group (10.3+/-3.1 vs 39.4+/-8.6 vs 42.3+/-9.8 and 43.6+/-10.6, P<0.01). These findings demonstrated that the AF protein vaccine effectively inhibited tumor angiogenesis and growth via production of autoantibodies against self-FGFR1.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
hepatocellular carcinoma  IDA 25440478 RGD 
hepatocellular carcinoma  ISOFGFR1 (Homo sapiens)25440478; 25440478 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Fgfr1  (Fibroblast growth factor receptor 1)

Genes (Mus musculus)
Fgfr1  (fibroblast growth factor receptor 1)

Genes (Homo sapiens)
FGFR1  (fibroblast growth factor receptor 1)


Additional Information