RGD Reference Report - Lower succinyl-CoA:3-ketoacid-CoA transferase (SCOT) and ATP citrate lyase in pancreatic islets of a rat model of type 2 diabetes: knockdown of SCOT inhibits insulin release in rat insulinoma cells. - Rat Genome Database
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Lower succinyl-CoA:3-ketoacid-CoA transferase (SCOT) and ATP citrate lyase in pancreatic islets of a rat model of type 2 diabetes: knockdown of SCOT inhibits insulin release in rat insulinoma cells.

Authors: Hasan, NM  Longacre, MJ  Seed Ahmed, M  Kendrick, MA  Gu, H  Ostenson, CG  Fukao, T  MacDonald, MJ 
Citation: Hasan NM, etal., Arch Biochem Biophys. 2010 Jul;499(1-2):62-8. Epub 2010 May 9.
RGD ID: 2326185
Pubmed: (View Article at PubMed) PMID:20460097
DOI: Full-text: DOI:10.1016/j.abb.2010.05.007

Succinyl-CoA:3-ketoacid-CoA transferase (SCOT) is a mitochondrial enzyme that catalyzes the reversible transfer of coenzyme-A from acetoacetyl-CoA to succinate to form acetoacetate and succinyl-CoA. mRNAs of SCOT and ATP citrate lyase were decreased 55% and 58% and enzyme activities were decreased >70% in pancreatic islets of the GK rat, a model of type 2 diabetes. INS-1 832/13 cells were transfected with shRNAs targeting SCOT mRNA to generate cell lines with reduced SCOT activity. Two cell lines with >70% knockdown of SCOT activity showed >70% reduction in glucose- or methyl succinate-plus-beta-hydroxybutyrate-stimulated insulin release. Less inhibition of insulin release was observed with two cell lines with less knockdown of SCOT. Previous studies showed knockdown of ATP citrate lyase in INS-1 832/13 cells does not lower insulin release. The results further support work that suggests mitochondrial pathways involving SCOT which supply acetoacetate for export to the cytosol are important for insulin secretion.

Annotation

Disease Annotations    

Gene Ontology Annotations    

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Oxct1  (3-oxoacid CoA transferase 1)

Genes (Mus musculus)
Oxct1  (3-oxoacid CoA transferase 1)

Genes (Homo sapiens)
OXCT1  (3-oxoacid CoA-transferase 1)


Additional Information