RGD Reference Report - Preparation of a conjugate of mitomycin C and anti-neural cell adhesion molecule monoclonal antibody for specific chemotherapy against biliary tract carcinoma. - Rat Genome Database

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Preparation of a conjugate of mitomycin C and anti-neural cell adhesion molecule monoclonal antibody for specific chemotherapy against biliary tract carcinoma.

Authors: Tanaka, J  Sato, E  Saito, Y  Kusano, T  Koyama, K 
Citation: Tanaka J, etal., Surg Today. 1998;28(11):1217-20.
RGD ID: 2326076
Pubmed: PMID:9851639   (View Abstract at PubMed)

To develop a specific chemotherapy modality for the perineural invasion of neural cell adhesion molecule (NCAM) positive biliary tract cancer, an anticancer drug, mitomycin C (MMC), was covalently bound to anti-NCAM monoclonal antibody (anti-NCAM MoAb) to form a conjugate using the cyanogen bromide method. The substitution ratio of the conjugate determined spectrophotometrically was 3.5 (MMC mol/immunoglobulin G mol). The cytotoxic activity of the conjugate, which was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test in the growth inhibition of the neuroblastoma cell line with NCAM expression, was maintained at 65.8% to 94.5% compared with the same concentration of MMC solution. The binding activity of the conjugate to the NCAM-positive bile duct cancer was examined by immunohistochemical staining and proved to be the same level as that of free anti-NCAM MoAb. The conjugate prepared in this study therefore appeared to be a potentially useful tool for immunotargeting specific chemotherapy against biliary tract cancer with NCAM expression.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Bile Duct Neoplasms  IDA 2326076 RGD 
Bile Duct Neoplasms  ISONCAM1 (Homo sapiens)2326076; 2326076 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ncam1  (neural cell adhesion molecule 1)

Genes (Mus musculus)
Ncam1  (neural cell adhesion molecule 1)

Genes (Homo sapiens)
NCAM1  (neural cell adhesion molecule 1)


Additional Information