RGD Reference Report - Expression of pancreatic endocrine markers by prolactin-treated rat bone marrow mesenchymal stem cells. - Rat Genome Database

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Expression of pancreatic endocrine markers by prolactin-treated rat bone marrow mesenchymal stem cells.

Authors: Gonzalez, P  Santos, TM  Calil, A  Corradi Perini, C  Percegona, LS  Silva, IC  Kuligovski, C  Aguiar, AM  Camara, NO  Aita, CA 
Citation: Gonzalez P, etal., Transplant Proc. 2010 Mar;42(2):566-9.
RGD ID: 2326021
Pubmed: PMID:20304194   (View Abstract at PubMed)
DOI: DOI:10.1016/j.transproceed.2010.01.031   (Journal Full-text)

BACKGROUND: Mesenchymal stem cells (MSCs) are an attractive source for generation of cells with beta-cell properties. Previous studies have demonstrated the ability of prolactin to induce an increase in beta-cell mass and maturation, which suggests beneficial effects of its use in MSC differentiation protocols. OBJECTIVE: To evaluate the expression of endocrine differentiation markers in rat MSCs treated in vitro with prolactin. METHODS: Mesenchymal stem cells from bone marrow of Wistar rats were isolated, expanded, and characterized. Differentiation of MSCs was induced in medium containing 23 mmol/L of glucose, and nicotinamide, 2-mercaptoethanol, and exendin-4, in the presence or absence of 500 ng/mL of rat recombinant prolactin. Expression of endocrine markers and prolactin receptor genes was evaluated using real-time polymerase chain reaction, and compared between culture stages and presence vs absence of prolactin in the culture medium. Expression of insulin, somatostatin, glucagon, and pancreatic and duodenal homeobox 1 was also evaluated at immunofluorescence microscopy. RESULTS: Isolated cells were mostly MSCs, as confirmed at fluorescent-activated cell sorting and cytochemistry. Pax6, Ngn-3, Isl1, NeuroD1, Nkx2.2, and Nkx6.1 exhibited varied expression during culture stages. The long form of the prolactin receptor messenger RNA was induced in prolactin-treated cultures (P < .05). The somatostatin gene was induced in early stages of differentiation (P < .05), and its expression was induced by prolactin, as confirmed using immunofluorescence. CONCLUSION: Culture of rat bone marrow MSCs in differentiation medium induces expression of pancreatic endocrine-specific genes, and somatostatin and prolactin receptor expression was also induced by prolactin.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to peptide hormone stimulus  IEP 2326021prolactinRGD 

Objects Annotated

Genes (Rattus norvegicus)
Nkx6-1  (NK6 homeobox 1)


Additional Information