RGD Reference Report - Elevated caspase and AIF gene expression correlate with progression of sarcopenia during aging in male F344BN rats. - Rat Genome Database

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Elevated caspase and AIF gene expression correlate with progression of sarcopenia during aging in male F344BN rats.

Authors: Baker, DJ  Hepple, RT 
Citation: Baker DJ and Hepple RT, Exp Gerontol. 2006 Nov;41(11):1149-56. Epub 2006 Oct 6.
RGD ID: 2325745
Pubmed: PMID:17029665   (View Abstract at PubMed)
DOI: DOI:10.1016/j.exger.2006.08.007   (Journal Full-text)

To establish the relationship between the progression of sarcopenia and apoptosis we examined apoptotic gene expression in plantaris muscles (Pl) from 8 mo old (n=8), 30 mo old (n=8) and 35 mo old (n=6) male rats by real-time PCR. Pl mass declined from 368 +/- 7 mg at 8 mo to 333 +/- 7 mg at 30 mo (P<0.05) and 210 +/- 15 mg at 35 mo of age (P<0.05). BAX, Bcl-2, and Apaf-1 expression decreased by 62-74% at 30 mo and by 90-96% at 35 mo of age (all P<0.05 vs 8 mo old). In contrast, the expression of Caspases 3, 8, and 9 and AIF increased 3- to 5-fold at 30 mo (NS) and 7- to 50-fold at 35 mo of age (P<0.05). There were significant (P<0.05) correlations between Pl mass and Caspase 3 (r(2)=-0.60), Caspase 9 (r(2)=-0.58), Caspase 8 (r(2)=-0.50), and AIF (r(2)=-0.48). Thus, our results show that the expression of some genes involved in apoptosis increase with aging in Pl and correlate with progression of sarcopenia (Caspase 3, Caspase 9, Caspase 8, and AIF), whereas others decline with aging (BAX, Bcl-2, and Apaf-1).

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
muscular atrophy  ISOApaf1 (Rattus norvegicus)2325745; 2325745mRNA:decreased expression:skeletal muscle tissueRGD 
muscular atrophy  IEP 2325745mRNA:decreased expression:skeletal muscle tissueRGD 
Sarcopenia severityISOAifm1 (Rattus norvegicus)2325745; 2325745RNA:increased expression:plantaris muscle:RGD 
Sarcopenia severityIEP 2325745RNA:increased expression:plantaris muscle:RGD 
Sarcopenia  ISOBax (Rattus norvegicus)2325745; 2325745mRNA:decreased expression:plantarisRGD 
Sarcopenia  ISOBcl2 (Rattus norvegicus)2325745; 2325745mRNA:decreased expression:plantarisRGD 
Sarcopenia  IEP 2325745; 2325745mRNA:decreased expression:plantarisRGD 

Objects Annotated

Genes (Rattus norvegicus)
Aifm1  (apoptosis inducing factor, mitochondria associated 1)
Apaf1  (apoptotic peptidase activating factor 1)
Bax  (BCL2 associated X, apoptosis regulator)
Bcl2  (BCL2, apoptosis regulator)

Genes (Mus musculus)
Aifm1  (apoptosis-inducing factor, mitochondrion-associated 1)
Apaf1  (apoptotic peptidase activating factor 1)
Bax  (BCL2-associated X protein)
Bcl2  (B cell leukemia/lymphoma 2)

Genes (Homo sapiens)
AIFM1  (apoptosis inducing factor mitochondria associated 1)
APAF1  (apoptotic peptidase activating factor 1)
BAX  (BCL2 associated X, apoptosis regulator)
BCL2  (BCL2 apoptosis regulator)


Additional Information