RGD Reference Report - Up-regulation of annexin A2 in cholangiocarcinoma caused by Opisthorchis viverrini and its implication as a prognostic marker. - Rat Genome Database

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Up-regulation of annexin A2 in cholangiocarcinoma caused by Opisthorchis viverrini and its implication as a prognostic marker.

Authors: Yonglitthipagon, P  Pairojkul, C  Chamgramol, Y  Mulvenna, J  Sripa, B 
Citation: Yonglitthipagon P, etal., Int J Parasitol. 2010 May 19.
RGD ID: 2325728
Pubmed: PMID:20493868   (View Abstract at PubMed)
PMCID: PMC3739719   (View Article at PubMed Central)
DOI: DOI:10.1016/j.ijpara.2010.05.002   (Journal Full-text)

Cholangiocarcinoma (CCA), or cancer of the bile ducts, is primarily associated with infection with the liver fluke Opisthorchis viverrini in northeast Thailand. The disease is associated with late presentation, poses challenges for diagnosis and has a high mortality rate - features that highlight the need for tumor markers. At present, there are no specific tumor markers that can indicate the early stages and status of CCA. Proteomic analysis of the proteins expressed on the surface of tumor cells is particularly difficult since proteome-wide analysis of surface membrane proteins has thus far been hampered by the lack of effective strategies to profile hydrophobic membrane proteins. In this study, a sequential protein extraction was utilized to overcome this problem. Membrane protein was extracted from four CCA cell lines with different tumor forming capabilities. The non-tumor H69 biliary cell line was used as a control. Two-dimensional-PAGE followed by MALDI-TOF-MS was used to identify differentially expressed proteins. Among 20 up-regulated membrane proteins identified in the CCA cell lines was ANXA2, a participant in tumor invasion and metastasis in other cancers. Accordingly, ANXA2 was verified in human subjects by probing, using a commercial anti-mouse monoclonal antibody and a tissue microarray of CCA (301 diagnosed cases), where it was found to associate with one of several tumor progression stages as reflected by lymphatic invasion (P = 0.014) and metastasis (P = 0.026). Patients with high expression of ANXA2 had a significantly shorter survival time (P = 0.011). ANXA2 expression in tumors may be useful for predicting the poor outcome of CCA patients.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cholangiocarcinoma disease_progressionIEP 2325728 RGD 
cholangiocarcinoma disease_progressionISOANXA2 (Homo sapiens)2325728; 2325728 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Anxa2  (annexin A2)

Genes (Mus musculus)
Anxa2  (annexin A2)

Genes (Homo sapiens)
ANXA2  (annexin A2)


Additional Information