RGD Reference Report - [Effects of electromagnetic pulse on blood-brain barrier permeability and tight junction proteins in rats] - Rat Genome Database

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[Effects of electromagnetic pulse on blood-brain barrier permeability and tight junction proteins in rats]

Authors: Qiu, LB  Ding, GR  Zhang, YM  Zhou, Y  Wang, XW  Li, KC  Xu, SL  Tan, J  Zhou, JX  Guo, GZ 
Citation: Qiu LB, etal., Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2009 Sep;27(9):539-43.
RGD ID: 2325126
Pubmed: PMID:20137299   (View Abstract at PubMed)

OBJECTIVE: To study the effect of electromagnetic pulse (EMP) on the permeability of blood-brain barrier, tight junction (TJ)-associated protein expression and localization in rats. METHODS: 66 male SD rats, weighing (200 approximately 250) g, were sham or whole-body exposed to EMP at 200 kV/m for 200 pulses. The repetition rate was 1 Hz. The permeability of the blood-brain barrier in rats was assessed by albumin immunohistochemistry. The expression of typical tight junction protein ZO-1 and occludin in both cerebral cortex homogenate and cerebral cortex microvessel homogenate was analyzed by the Western blotting and the distribution of ZO-1 and occludin was examined by immunofluorescence microscopy. RESULTS: In the sham exposure rats, no brain capillaries showed albumin leakage, at 0.5 h after 200 kV/m EMP exposure for 200 pulses; a few brain capillaries with extravasated serum albumin was found, with the time extended, the number of brain capillaries with extravasated serum albumin increased, and reached the peak at 3 h, then began to recover at 6 h. In addition, no change in the distribution of the occludin was found after EMP exposure. Total occludin expression had no significant change compared with the control. However, the expression level of ZO-1 significantly decreased at 1 h and 3 h after EMP exposure in both cerebral cortex homogenate and cerebral cortex microvessel homogenate. Furthermore, immunofluorescence studies also showed alterations in ZO-1 protein localization in cerebral cortex microvessel. CONCLUSION: The EMP exposure (200 kV/m, 200 pulses) could increase blood-brain barrier permeability in rat, and this change is associated with specific alterations in tight junction protein ZO-1.



Gene Ontology Annotations    

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Tjp1  (tight junction protein 1)


Additional Information