RGD Reference Report - Neuroprotective gene expression profiles in ischemic cortical cultures preconditioned with IGF-1 or bFGF. - Rat Genome Database

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Neuroprotective gene expression profiles in ischemic cortical cultures preconditioned with IGF-1 or bFGF.

Authors: Yoshida, E  Atkinson, TG  Chakravarthy, B 
Citation: Yoshida E, etal., Brain Res Mol Brain Res. 2004 Nov 24;131(1-2):33-50.
RGD ID: 2324642
Pubmed: PMID:15530650   (View Abstract at PubMed)
DOI: DOI:10.1016/j.molbrainres.2004.08.023   (Journal Full-text)

The mechanisms underlying growth factor preconditioning of neurons are only partially elucidated, and no studies have been conducted in this area using a gene profiling approach. We used cDNA microarrays to compare the transcriptional profiles of cells preconditioned either with insulin-like growth factor I (IGF-1) or basic fibroblast growth factor (bFGF), to identify differentially regulated genes that may function in growth factor signaling, response to oxygen-glucose deprivation (OGD), and most importantly, cell survival. Primary rat cortical cultures were treated with bFGF or IGF-1 for 2, 24, or 24 h followed by OGD for 90 min, and compared with cells that were subject to OGD without growth factor pretreatment. Although the majority of surveyed genes were unchanged in all experimental treatments, 175 genes (10% of the cDNAs on the chip) were found to be differentially regulated in at least one of the treatment conditions. Hierarchical clustering of these 175 genes was used to identify four expression clusters: IGF-1 regulated, bFGF regulated, OGD regulated, and putative neuroprotective genes. Further analysis using realtime RT-PCR confirmed that we had identified genes that are regulated by single growth factors, as well as several more that are co-regulated by both IGF-1 and bFGF. These genes can influence neuronal survival by affecting diverse pathways such as growth factor signal transduction (CD44, DTR, DUSP6, EPS8, IGFBP3), DNA repair and transcription (FOXJ1), metabolic homeostasis (RASA1, SHMT2), cytoskeletal stability (MSN, MAPT) and cholesterol biosynthesis (FDFT1, FDPS).

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to fibroblast growth factor stimulus  IEP 2324642 RGD 
cellular response to insulin-like growth factor stimulus  IEP 2324642 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Foxj1  (forkhead box J1)
Rasa1  (RAS p21 protein activator 1)


Additional Information