RGD Reference Report - The role of LPA1 in formation of synapses among cultured hippocampal neurons. - Rat Genome Database

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The role of LPA1 in formation of synapses among cultured hippocampal neurons.

Authors: Pilpel, Y  Segal, M 
Citation: Pilpel Y and Segal M, J Neurochem. 2006 Jun;97(5):1379-92. Epub 2006 Apr 21.
RGD ID: 2317707
Pubmed: PMID:16638019   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1471-4159.2006.03825.x   (Journal Full-text)

We studied the lysophosphatidic acid receptor-1 (LPA1) gene, which we found to be expressed endogenously in cultured hippocampal neurons, and in vivo in young (1-week-old) rat brain slices. Overexpressed green fluorescent protein (GFP)-tagged, membrane-associated LPA1 accumulated in a punctate manner over the entire dendritic tree and caused an increase in dendritic spine density. About half of the dendritic spines in the LPA1-transfected neurons displayed distinct fluorescent puncta, and this subset of spines was also substantially larger than puncta-free, LPA1-transfected or control GFP spines. This phenotype could also be seen in cells transfected with a ligand-binding, defective mutant and is therefore not dependent on interaction with an ambient ligand. While spontaneous miniature excitatory synaptic currents were of the same amplitudes, they decayed slower in LPA1-transfected neurons compared with GFP controls. We propose that LPA1 may play a role in the formation and modulation of the dendritic spine synapse.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of dendritic spine development  IDA 2317707 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
dendritic shaft  IDA 2317707 RGD 
dendritic spine  IDA 2317707 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Lpar1  (lysophosphatidic acid receptor 1)


Additional Information