RGD Reference Report - Gene expression profiling of microdissected pancreatic ductal carcinomas using high-density DNA microarrays. - Rat Genome Database

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Gene expression profiling of microdissected pancreatic ductal carcinomas using high-density DNA microarrays.

Authors: Grutzmann, R  Pilarsky, C  Ammerpohl, O  Luttges, J  Bohme, A  Sipos, B  Foerder, M  Alldinger, I  Jahnke, B  Schackert, HK  Kalthoff, H  Kremer, B  Kloppel, G  Saeger, HD 
Citation: Grutzmann R, etal., Neoplasia. 2004 Sep-Oct;6(5):611-22.
RGD ID: 2317697
Pubmed: PMID:15548371   (View Abstract at PubMed)
PMCID: PMC1531666   (View Article at PubMed Central)
DOI: DOI:10.1593/neo.04295   (Journal Full-text)

Pancreatic ductal adenocarcinoma (PDAC) remains an important cause of malignancy-related death and is the eighth most common cancer with the lowest overall 5-year relative survival rate. To identify new molecular markers and candidates for new therapeutic regimens, we investigated the gene expression profile of microdissected cells from 11 normal pancreatic ducts, 14 samples of PDAC, and 4 well-characterized pancreatic cancer cell lines using the Affymetrix U133 GeneChip set. RNA was extracted from microdissected samples and cell lines, amplified, and labeled using a repetitive in vitro transcription protocol. Differentially expressed genes were identified using the significance analysis of microarrays program. We found 616 differentially expressed genes. Within these, 140 were also identified in PDAC by others, such as Galectin-1, Galectin-3, and MT-SP2. We validated the differential expression of several genes (e.g., CENPF, MCM2, MCM7, RAMP, IRAK1, and PTTG1) in PDAC by immunohistochemistry and reverse transcription polymerase chain reaction. We present a whole genome expression study of microdissected tissues from PDAC, from microdissected normal ductal pancreatic cells and pancreatic cancer cell lines using high-density microarrays. Within the panel of genes, we identified novel differentially expressed genes, which have not been associated with the pathogenesis of PDAC before.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
pancreatic ductal carcinoma  IEP 2317697mRNA and protein:increased expression:pancreatic ductRGD 
pancreatic ductal carcinoma  ISOMCM7 (Homo sapiens)2317697; 2317697mRNA and protein:increased expression:pancreatic ductRGD 

Objects Annotated

Genes (Rattus norvegicus)
Mcm7  (minichromosome maintenance complex component 7)

Genes (Mus musculus)
Mcm7  (minichromosome maintenance complex component 7)

Genes (Homo sapiens)
MCM7  (minichromosome maintenance complex component 7)


Additional Information