RGD Reference Report - Global gene expression profiling of renal scarring in a rat model of pyelonephritis. - Rat Genome Database

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Global gene expression profiling of renal scarring in a rat model of pyelonephritis.

Authors: Ichino, M  Mori, T  Kusaka, M  Kuroyanagi, Y  Ishikawa, K  Shiroki, R  Kowa, H  Kurahashi, H  Hoshinaga, K 
Citation: Ichino M, etal., Pediatr Nephrol. 2008 Jul;23(7):1059-71. Epub 2008 Jan 23.
RGD ID: 2317665
Pubmed: PMID:18214547   (View Abstract at PubMed)
DOI: DOI:10.1007/s00467-007-0717-6   (Journal Full-text)

Renal scarring is a serious complication of chronic pyelonephritis that occurs due to vesicoureteral reflux. In our study, we performed global expression profiling of the kidney during renal scarring formation in a rat pyelonephritis model. An inoculum of Escherichia coli was injected directly into the renal cortex. Histologically, renal scarring developed during the 3-to-4 week period after injection. The time-course expression profile of 18,442 genes was then analyzed using microarrays, followed by validation with real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Most of the genes found to be up-regulated during renal scarring are associated with immune and defense responses, including cytokines, chemokines and their receptors, complement factors, adhesion molecules and extracellular matrix proteins. These genes were up-regulated as early as 1 week after injection, when no fibrotic changes were yet evident, peaked at 2 weeks, and gradually decreased thereafter. However, a subset of cytokine genes was found to be persistently activated even at 6 weeks after injection, including interleukin (IL)-1beta, transforming growth factor (TGF)-beta, and IL-3. Further statistical analysis indicated that the pathways mediated by these cytokines are activated concomitantly with renal scarring formation. The products of these genes may thus potentially be novel non-invasive diagnostic or prognostic biomarkers of renal scarring.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
IL3Humanpyelonephritis  ISOIl3 (Rattus norvegicus)mRNA:increased expression:kidneyRGD 
Il3Ratpyelonephritis  IEP mRNA:increased expression:kidneyRGD 
Il3Mousepyelonephritis  ISOIl3 (Rattus norvegicus)mRNA:increased expression:kidneyRGD 

Objects Annotated

Genes (Rattus norvegicus)
Il3  (interleukin 3)

Genes (Mus musculus)
Il3  (interleukin 3)

Genes (Homo sapiens)
IL3  (interleukin 3)


Additional Information