RGD Reference Report - Mammalian mitochondrial transfer RNAs: chromatographic properties, size and origin. - Rat Genome Database

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Mammalian mitochondrial transfer RNAs: chromatographic properties, size and origin.

Authors: Aujame, L  Freeman, KB 
Citation: Aujame L and Freeman KB, Nucleic Acids Res. 1979 Feb;6(2):455-69.
RGD ID: 2317000
Pubmed: PMID:424302   (View Abstract at PubMed)
PMCID: PMC327707   (View Article at PubMed Central)

Incubation of isolated rat liver mitochondria with radioactive amino acids resulted in the charging of tRNAs for arginine, asparagine, leucine, lysine, methionine, proline and valine. The aminoacyl-tRNAs were shown to be distinct from their cytosolic counterparts by chromatography on RPC-5. By electrophoresis on urea polyacrylamide slab gels it was found that all these mitochondrial aminoacyl-tRNAs were about 70-76 nucleotides long. The unique mitochondrial asparaginyl- and prolyl-tRNAs, not previously identified in mammalian cells, were shown to hybridize to mtDNA. Mitochondrial leucyl-tRNA separated into 3 peaks on RPC-5 and the first species was shown to be different than a combination of the other two by molecular size and partial RNase T1 digestion patterns. Each was coded by a separate gene on mtDNA as shown by partial additivity of hybridization. Separate genes for mitochondrial tRNAMetm and tRNAMetf, separated by RPC-5 chromatography, were also demonstrated. These results bring to 21 the number of individual tRNAs coded by mammalian mtDNA.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
mitochondrial translational elongation  IDA 2317000 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
CCC codon-amino acid adaptor activity  IDA 2317000 RGD 

Objects Annotated

Genes (Rattus norvegicus)
mt-Tp  (mitochondrially encoded tRNA proline)


Additional Information