RGD Reference Report - Lanosterol 14 alpha-demethylase (cytochrome P-45014DM): modulation of its enzyme activity by cholesterol feeding. - Rat Genome Database

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Lanosterol 14 alpha-demethylase (cytochrome P-45014DM): modulation of its enzyme activity by cholesterol feeding.

Authors: Sonoda, Y  Amano, C  Endo, M  Sato, Y  Sekigawa, Y  Fukuhara, M 
Citation: Sonoda Y, etal., Biol Pharm Bull. 1995 Jul;18(7):1009-11.
RGD ID: 2316902
Pubmed: PMID:7581240   (View Abstract at PubMed)

Regulation of the activity of lanosterol 14 alpha-demethylase (cytochrome P-45014DM) by dietary cholesterol was studied in rats. In male rats fed a 3% cholesterol diet for 1 and 4 weeks, the activity of 24,25-dihydrolanosterol 14 alpha-demethylase was decreased in the liver. The cytochrome P-45014DM protein content detected by immunoblotting was also decreased by cholesterol feeding. These results demonstrate that dietary cholesterol acts as a repressive factor for lanosterol 14 alpha-demethylase. Further, the activity was enhanced by preincubation with phosphatase of the enzyme solution from both the control and cholesterol fed rats at the same rate. This result suggests that regulation of the activity involves phosphorylation of this enzyme.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Cyp51Ratcholesterol biosynthetic process via 24,25-dihydrolanosterol  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Cyp51Ratsterol 14-demethylase activity  IDA  RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations


  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CYP51A1Humancholesterol biosynthetic pathway   ISOCyp51 (Rattus norvegicus) RGD 
Cyp51Ratcholesterol biosynthetic pathway   IDA  RGD 
Cyp51Mousecholesterol biosynthetic pathway   ISOCyp51 (Rattus norvegicus) RGD 
Objects Annotated

Genes (Rattus norvegicus)
Cyp51  (cytochrome P450, family 51)

Genes (Mus musculus)
Cyp51  (cytochrome P450, family 51)

Genes (Homo sapiens)
CYP51A1  (cytochrome P450 family 51 subfamily A member 1)


Additional Information