RGD Reference Report - Ultrastructural localisation and differential agonist-induced regulation of AMPA and kainate receptors present at the presynaptic active zone and postsynaptic density.

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Ultrastructural localisation and differential agonist-induced regulation of AMPA and kainate receptors present at the presynaptic active zone and postsynaptic density.
Authors:	Feligioni, M Holman, D Haglerod, C Davanger, S Henley, JM
Citation:	Feligioni M, etal., J Neurochem. 2006 Oct;99(2):549-60. Epub 2006 Aug 11.
RGD ID:	2316535
Pubmed:	PMID:16903873 (View Abstract at PubMed)
PMCID:	PMC3314506 (View Article at PubMed Central)
DOI:	DOI:10.1111/j.1471-4159.2006.04087.x (Journal Full-text)
Activity-dependent changes in ionotropic glutamate receptors at the postsynaptic membrane are well established and this regulation plays a central role in the expression of synaptic plasticity. However, very little is known about the distributions and regulation of ionotropic receptors at presynaptic sites. To determine if presynaptic receptors are subject to similar regulatory processes we investigated the localisation and modulation of AMPA (GluR1, GluR2, GluR3) and kainate (GluR6/7, KA2) receptor subunits by ultrasynaptic separation and immunoblot analysis of rat brain synaptosomes. All of the subunits were enriched in the postsynaptic fraction but were also present in the presynaptic and non-synaptic synaptosome fractions. AMPA stimulation resulted in a marked decrease in postsynaptic GluR2 and GluR3 subunits, but an increase in GluR6/7. Conversely, GluR2 and GluR3 increased in the presynaptic fraction whereas GluR6/7 decreased. There were no significant changes in any of the compartments for GluR1. NMDA treatment decreased postsynaptic GluR1, GluR2 and GluR6/7 but increased presynaptic levels of these subunits. NMDA treatment did not evoke changes in GluR3 localisation. Our results demonstrate that presynaptic and postsynaptic subunits are regulated in opposite directions by AMPA and NMDA stimulation.

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Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
Gria1	Rat	postsynaptic membrane	is_active_in	IEP		PMID:16903873	SynGO
Gria1	Rat	postsynaptic membrane	is_active_in	IDA		PMID:16903873	SynGO
Gria2	Rat	postsynaptic membrane	is_active_in	IEP		PMID:16903873	SynGO
Gria2	Rat	postsynaptic membrane	is_active_in	IDA		PMID:16903873	SynGO
Gria3	Rat	postsynaptic membrane	is_active_in	IEP		PMID:16903873	SynGO
Gria3	Rat	postsynaptic membrane	is_active_in	IDA		PMID:16903873	SynGO
Grik5	Rat	postsynaptic membrane	is_active_in	IDA		PMID:16903873	SynGO
Grik5	Rat	postsynaptic membrane	is_active_in	IEP		PMID:16903873	SynGO
Gria1	Rat	presynaptic membrane	is_active_in	IDA		PMID:16903873	SynGO
Gria1	Rat	presynaptic membrane	is_active_in	IEP		PMID:16903873	SynGO
Gria2	Rat	presynaptic membrane	is_active_in	IDA		PMID:16903873	SynGO
Gria2	Rat	presynaptic membrane	is_active_in	IEP		PMID:16903873	SynGO
Gria3	Rat	presynaptic membrane	is_active_in	IDA		PMID:16903873	SynGO
Gria3	Rat	presynaptic membrane	is_active_in	IEP		PMID:16903873	SynGO
Grik5	Rat	presynaptic membrane	is_active_in	IDA		PMID:16903873	SynGO
Grik5	Rat	presynaptic membrane	is_active_in	IEP		PMID:16903873	SynGO

Objects Annotated

Genes (Rattus norvegicus)

Gria1 (glutamate ionotropic receptor AMPA type subunit 1)

Gria2 (glutamate ionotropic receptor AMPA type subunit 2)

Gria3 (glutamate ionotropic receptor AMPA type subunit 3)

Grik5 (glutamate ionotropic receptor kainate type subunit 5)

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Ultrastructural localisation and differential agonist-induced regulation of AMPA and kainate receptors present at the presynaptic active zone and postsynaptic density.