RGD Reference Report - Cdc2-mediated Schwann cell migration during peripheral nerve regeneration. - Rat Genome Database

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Cdc2-mediated Schwann cell migration during peripheral nerve regeneration.

Authors: Han, IS  Seo, TB  Kim, KH  Yoon, JH  Yoon, SJ  Namgung, U 
Citation: Han IS, etal., J Cell Sci. 2007 Jan 15;120(Pt 2):246-55. Epub 2007 Jan 2.
RGD ID: 2316482
Pubmed: PMID:17200138   (View Abstract at PubMed)
DOI: DOI:10.1242/jcs.03322   (Journal Full-text)

Schwann cell migration facilitates peripheral nerve regeneration after injury. We have recently found increased activation of Cdc2 kinase in regenerating sciatic nerves. Here we show that Cdc2 phosphorylation of caldesmon regulates Schwann cell migration and nerve regeneration. A robust but transient increase in Cdc2 expression was found in cultured Schwann cells prepared from the sciatic nerve in rats that had undergone crush injury for 7 days. These ;injury-preconditioned' Schwann cells exhibited enhanced migration compared with non-preconditioned control cells and treatment with the cdk inhibitor roscovitine prevented cell migration. After transduction with recombinant Cdc2 DNA adenoviral vectors, Schwann cells were implanted into sciatic nerves; those expressing wild-type Cdc2 migrated further in the distal direction than those expressing dominant-negative Cdc2. We identified caldesmon as a downstream substrate of Cdc2 in Schwann cells and its phosphorylation by Cdc2 changed its subcellular localization. Overexpression of dominant-negative caldesmon significantly counteracted the migration effect caused by Cdc2. Finally, neurite outgrowth of cultured DRG sensory neurons, facilitated by co-culture with injury-preconditioned Schwann cells, was suppressed by roscovitine treatment. The results indicate that activation of the Cdc2-caldesmon pathway is necessary for Schwann cell migration and suggest a role for this pathway in peripheral axonal growth.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Cdk1Ratresponse to axon injury  IEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cdk1  (cyclin-dependent kinase 1)


Additional Information