RGD Reference Report - Farnesyl-diphosphate synthase is localized in peroxisomes. - Rat Genome Database

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Farnesyl-diphosphate synthase is localized in peroxisomes.

Authors: Krisans, SK  Ericsson, J  Edwards, PA  Keller, GA 
Citation: Krisans SK, etal., J Biol Chem. 1994 May 13;269(19):14165-9.
RGD ID: 2316305
Pubmed: PMID:8188698   (View Abstract at PubMed)

In this study, we have investigated the subcellular localization of farnesyl-diphosphate synthase (FPP synthase). FPP synthase produces FPP, which is utilized in the synthesis of squalene, cholesterol, farnesylated and geranylgeranylated proteins, dolichols, coenzyme Q, and the isoprenoid moiety of heme a. This enzyme is found in the 100,000 x g supernatant fraction of cells or tissues and has been considered to be a cytoplasmic protein. In this study, analysis of FPP synthase activity and protein in fractionated rat liver together with immunofluorescent and immunoelectron microscopy studies demonstrated unequivocally that FPP synthase is largely localized in peroxisomes. These data, in combination with the previous observation that mevalonate kinase is predominantly localized in peroxisomes, suggest that peroxisomes are the major site of synthesis of FPP from mevalonate. We also demonstrate that in liver tissue obtained from patients with peroxisomal deficiency diseases (Zellweger syndrome and neonatal adrenoleukodystrophy), the activities of five enzymes involved in isoprenoid synthesis, namely mevalonate kinase, phosphomevalonate kinase, mevalonate-diphosphate decarboxylase, isopentenyl-diphosphate isomerase, and FPP synthase, are significantly reduced, consistent with a peroxisomal localization of these enzymes.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
FdpsRatfarnesyl diphosphate biosynthetic process  IDA  RGD 

Cellular Component

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
FdpsRatperoxisome  IDA  RGD 

Molecular Function

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
FdpsRatgeranyltranstransferase activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Fdps  (farnesyl diphosphate synthase)

Additional Information