RGD Reference Report - Expression and regulation of antimicrobial peptide rCRAMP after bacterial infection in primary rat meningeal cells. - Rat Genome Database

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Expression and regulation of antimicrobial peptide rCRAMP after bacterial infection in primary rat meningeal cells.

Authors: Brandenburg, LO  Varoga, D  Nicolaeva, N  Leib, SL  Podschun, R  Wruck, CJ  Wilms, H  Lucius, R  Pufe, T 
Citation: Brandenburg LO, etal., J Neuroimmunol. 2009 Dec 10;217(1-2):55-64. Epub 2009 Oct 30.
RGD ID: 2316237
Pubmed: PMID:19879657   (View Abstract at PubMed)
DOI: DOI:10.1016/j.jneuroim.2009.10.004   (Journal Full-text)

Bacterial meningitis is characterized by an inflammation of the meninges and continues to be an important cause of mortality and morbidity. Meningeal cells cover the cerebral surface and are involved in the first interaction between pathogens and the brain. Little is known about the role of meningeal cells and the expression of antimicrobial peptides in the innate immune system. In this study we characterized the expression, secretion and bactericidal properties of rat cathelin-related antimicrobial peptide (rCRAMP), a homologue of the human LL-37, in rat meningeal cells after incubation with different bacterial supernatants and the bacterial cell wall components lipopolysaccharide (LPS) and peptidoglycan (PGN). Using an agar diffusion test, we observed that supernatants from meningeal cells incubated with bacterial supernatants, LPS and PGN showed signs of antimicrobial activity. The inhibition of rCRAMP expression using siRNA reduced the antimicrobial activity of the cell culture supernatants. The expression of rCRAMP in rat meningeal cells involved various signal transduction pathways and was induced by the inflammatory cytokines interleukin-1, -6 and tumor necrosis factor alpha. In an experimental model of meningitis, infant rats were intracisternally infected with Streptococcus pneumoniae and rCRAMP was localized in meningeal cells using immunohistochemistry. These results suggest that cathelicidins produced by meningeal cells play an important part in the innate immune response against pathogens in CNS bacterial infections.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Pneumococcal Meningitis  ISOCamp (Rattus norvegicus)2316237; 2316237protein:increased expression:brain and coronal section (rat)RGD 
Pneumococcal Meningitis  IEP 2316237protein:increased expression:brain and coronal section (rat)RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cell projection  IDA 2316237 RGD 
extracellular space  IDA 2316237 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Camp  (cathelicidin antimicrobial peptide)

Genes (Mus musculus)
Camp  (cathelicidin antimicrobial peptide)

Genes (Homo sapiens)
CAMP  (cathelicidin antimicrobial peptide)

Objects referenced in this article
Gene Camlg calcium modulating ligand Rattus norvegicus

Additional Information