RGD Reference Report - Cav3.1 (alpha1G) controls von Willebrand factor secretion in rat pulmonary microvascular endothelial cells. - Rat Genome Database

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Cav3.1 (alpha1G) controls von Willebrand factor secretion in rat pulmonary microvascular endothelial cells.

Authors: Zhou, C  Chen, H  Lu, F  Sellak, H  Daigle, JA  Alexeyev, MF  Xi, Y  Ju, J  Van Mourik, JA  Wu, S 
Citation: Zhou C, etal., Am J Physiol Lung Cell Mol Physiol. 2007 Apr;292(4):L833-44. Epub 2006 Dec 15.
RGD ID: 2316206
Pubmed: PMID:17172292   (View Abstract at PubMed)
PMCID: PMC3048959   (View Article at PubMed Central)
DOI: DOI:10.1152/ajplung.00377.2006   (Journal Full-text)

The T-type Ca2+ channel Cav3.1 subunit is present in pulmonary microvascular endothelial cells (PMVECs), but not in pulmonary artery endothelial cells (PAECs). The present study sought to assess the role of Cav3.1 in thrombin-induced Weibel-Palade body exocytosis and consequent von Willebrand factor (VWF) release. In PMVECs and PAECs transduced with a green fluorescent protein (GFP)-tagged VWF chimera, we examined the real-time dynamics and secretory process of VWF-GFP-containing vesicles in response to thrombin and the cAMP-elevating agent isoproterenol. Whereas thrombin stimulated a progressive decrease in the number of VWF-GFP-containing vesicles in both cell types, isoproterenol only decreased the number of VWF-GFP-containing vesicles in PAECs. In PMVECs, thrombin-induced decrease in the number of VWF-GFP-containing vesicles was nearly abolished by the T-type Ca2+ channel blocker mibefradil as well as by Cav3.1 gene silencing with small hairpin RNA. Expression of recombinant Cav3.1 subunit in PAECs resulted in pronounced increase in thrombin-stimulated Ca2+ entry, which is sensitive to mibefradil. Together, these data indicate that VWF secretion from lung endothelial cells is regulated by two distinct pathways involving Ca2+ or cAMP, and support the hypothesis that activation of Cav3.1 T-type Ca2+ channels in PMVECs provides a unique cytosolic Ca2+ source important for Gq-linked agonist-induced VWF release.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of calcium ion-dependent exocytosis  IMP 2316206 RGD 
regulation of calcium ion transport  IMP 2316206 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cacna1g  (calcium voltage-gated channel subunit alpha1 G)


Additional Information