RGD Reference Report - Regulation of T-type Cav3.1 channels expression by synthetic glucocorticoid dexamethasone in neonatal cardiac myocytes. - Rat Genome Database

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Regulation of T-type Cav3.1 channels expression by synthetic glucocorticoid dexamethasone in neonatal cardiac myocytes.

Authors: BenMohamed, F  Ferron, L  Ruchon, Y  Gouadon, E  Renaud, JF  Capuano, V 
Citation: BenMohamed F, etal., Mol Cell Biochem. 2009 Jan;320(1-2):173-83. Epub 2008 Sep 27.
RGD ID: 2316203
Pubmed: PMID:18820838   (View Abstract at PubMed)
DOI: DOI:10.1007/s11010-008-9919-x   (Journal Full-text)

The effect of the dexamethasone (Dex) on the regulation of the T-type Ca(2+) channel expressions was investigated in primary cultures of neonatal rat ventricular myocytes. We found that Dex (1 microM) increases the T-type Ca(2+) current (I(CaT)) associated with an increase in Ca(v)3.1 mRNA amount. We isolated the upstream region from Ca(v)3.1 encoding gene and tested the activity of the promoter in transfected ventricular myocytes. We found a minimal Dex-responsive region that displayed putative glucocorticoid receptor (GR) and nuclear factor kappa-B (NFkappaB) targets. The GR selective antagonist, RU38486 (10 microM), nearly turned off the transcriptional activity of Ca(v)3.1 encoding gene, and an NFkappaB inhibitor, pyrrolodine dithiocarbonate (10 microM), completely abolished the Dex-induced mRNA increase. However, Dex-induced GR and NFkappaB synthesis and nuclear translocation were not timely related to Ca(v)3.1 mRNA increase. These results indicate that both GR and NFkappaB were necessary, but not sufficient, to trigger the increase in Ca(v)3.1 mRNA amount. This study showed the relationship between glucocorticoid and T-type channels up-regulation that may be involved in cardiac development and pathology.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to dexamethasone stimulus  IEP 2316203 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cacna1g  (calcium voltage-gated channel subunit alpha1 G)


Additional Information