RGD Reference Report - Type 1 angiotensin II receptor-associated protein ARAP1 binds and recycles the receptor to the plasma membrane. - Rat Genome Database

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Type 1 angiotensin II receptor-associated protein ARAP1 binds and recycles the receptor to the plasma membrane.

Authors: Guo, DF  Chenier, I  Tardif, V  Orlov, SN  Inagami, T 
Citation: Guo DF, etal., Biochem Biophys Res Commun. 2003 Oct 31;310(4):1254-65.
RGD ID: 2316188
Pubmed: PMID:14559250   (View Abstract at PubMed)

The carboxyl terminus of the type 1 angiotensin II receptor (AT(1)) plays an important role in receptor phosphorylation, desensitization, and internalization. The yeast two-hybrid system was employed to isolate proteins associated with the carboxyl terminal region of the AT(1A) receptor. In the present study, we report the isolation of a novel protein, ARAP1, which promotes recycling of AT(1A) to the plasma membrane in HEK-293 cells. ARAP1 cDNA encodes a 493-amino-acid protein and its mRNA is ubiquitously expressed in rat tissues. A complex of ARAP1 and AT(1A) was observed by immunoprecipitation and Western blotting in HEK-293 cells. In the presence of ARAP1, recycled AT(1A) showed a significant Ca(2+) release response to a second stimulation by Ang II 30 min after the first treatment. Immunocytochemical analysis revealed co-localization of recycled AT(1A) and ARAP1 in the plasma membrane 45 min after the initial exposure to Ang II. Taken together, these results indicate a role for ARAP1 in the recycling of the AT(1) receptor to the plasma membrane with presumable concomitant recovery of receptor signal functions.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Agtr1aRatpositive regulation of receptor recycling involved_inIDA PMID:14559250UniProt 
Arap1Ratpositive regulation of receptor recycling  IDA  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Agtr1aRatcytoplasmic vesicle located_inIDA PMID:14559250UniProt 
Agtr1aRatGolgi apparatus located_inIDA PMID:14559250UniProt 
Agtr1aRatplasma membrane located_inIDA PMID:14559250UniProt 
Agtr1aRatrecycling endosome  IDA  RGD 
Arap1Rattrans-Golgi network  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Agtr1aRatprotein binding  IPIArap1 (Rattus norvegicus) RGD 
Arap1Rattype 1 angiotensin receptor binding  IPIAgtr1a (Rattus norvegicus) RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations


  
Objects Annotated

Genes (Rattus norvegicus)
Agtr1a  (angiotensin II receptor, type 1a)
Arap1  (ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1)

Genes (Mus musculus)
Agtr1a  (angiotensin II receptor, type 1a)
Arap1  (ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1)

Genes (Homo sapiens)
AGTR1  (angiotensin II receptor type 1)
ARAP1  (ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1)


Additional Information