RGD Reference Report - Type 1 angiotensin II receptor-associated protein ARAP1 binds and recycles the receptor to the plasma membrane. - Rat Genome Database

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Type 1 angiotensin II receptor-associated protein ARAP1 binds and recycles the receptor to the plasma membrane.

Authors: Guo, DF  Chenier, I  Tardif, V  Orlov, SN  Inagami, T 
Citation: Guo DF, etal., Biochem Biophys Res Commun. 2003 Oct 31;310(4):1254-65.
RGD ID: 2316188
Pubmed: (View Article at PubMed) PMID:14559250

The carboxyl terminus of the type 1 angiotensin II receptor (AT(1)) plays an important role in receptor phosphorylation, desensitization, and internalization. The yeast two-hybrid system was employed to isolate proteins associated with the carboxyl terminal region of the AT(1A) receptor. In the present study, we report the isolation of a novel protein, ARAP1, which promotes recycling of AT(1A) to the plasma membrane in HEK-293 cells. ARAP1 cDNA encodes a 493-amino-acid protein and its mRNA is ubiquitously expressed in rat tissues. A complex of ARAP1 and AT(1A) was observed by immunoprecipitation and Western blotting in HEK-293 cells. In the presence of ARAP1, recycled AT(1A) showed a significant Ca(2+) release response to a second stimulation by Ang II 30 min after the first treatment. Immunocytochemical analysis revealed co-localization of recycled AT(1A) and ARAP1 in the plasma membrane 45 min after the initial exposure to Ang II. Taken together, these results indicate a role for ARAP1 in the recycling of the AT(1) receptor to the plasma membrane with presumable concomitant recovery of receptor signal functions.

Annotation

Gene Ontology Annotations    

Biological Process

Cellular Component

Molecular Function

Molecular Pathway Annotations    
Objects Annotated

Genes (Rattus norvegicus)
Agtr1a  (angiotensin II receptor, type 1a)
Arap1  (ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1)

Genes (Mus musculus)
Agtr1a  (angiotensin II receptor, type 1a)
Arap1  (ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1)

Genes (Homo sapiens)
AGTR1  (angiotensin II receptor type 1)
ARAP1  (ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1)


Additional Information