RGD Reference Report - Characterization of mesenchymal stem cells from rat bone marrow: ultrastructural properties, differentiation potential and immunophenotypic markers. - Rat Genome Database

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Characterization of mesenchymal stem cells from rat bone marrow: ultrastructural properties, differentiation potential and immunophenotypic markers.

Authors: Karaoz, E  Aksoy, A  Ayhan, S  Sariboyaci, AE  Kaymaz, F  Kasap, M 
Citation: Karaoz E, etal., Histochem Cell Biol. 2009 Nov;132(5):533-46. Epub 2009 Aug 14.
RGD ID: 2316153
Pubmed: PMID:19688349   (View Abstract at PubMed)
DOI: DOI:10.1007/s00418-009-0629-6   (Journal Full-text)

Bone marrow-derived mesenchymal stem cells (BM-MSCs) can differentiate into many lineages. Although the growing interest in BM-MSCs has led to a number of characterization studies, some important biochemical and immunohistochemical properties are still lacking. In this study, morphological and immunophenotypic properties of BM-MSCs were examined in detail. Differentiation potential and growth kinetics of adult rat BM-MSCs were also determined. Immunohistochemistry and RT-PCR results indicated that BM-MSCs expressed myogenic (desmin, myogenin, myosin IIa, and alpha-SMA), neurogenic (gamma-enolase, MAP2a,b, c-fos, nestin, GFAP and beta III tubulin), and osteogenic (osteonectin, osteocalcin, osteopontin, Runx-2, BMP-2, BMP-4 and type I collagen) markers without stimulation towards differentiation. These expression patterns indicated why these cells can easily differentiate into multiple lineages both in vitro and in vivo. Ultrastructural characteristics of rBM-MSCs showed more developed and metabolically active cells.

Objects referenced in this article
Gene Bglap bone gamma-carboxyglutamate protein Rattus norvegicus

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