RGD Reference Report - Expression of Slit2 and Robo1 after traumatic lesions of the rat spinal cord. - Rat Genome Database

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Expression of Slit2 and Robo1 after traumatic lesions of the rat spinal cord.

Authors: Liu, JB  Jiang, YQ  Gong, AH  Zhang, ZJ  Jiang, Q  Chu, XP 
Citation: Liu JB, etal., Acta Histochem. 2009 Sep 22.
RGD ID: 2316127
Pubmed: PMID:19783284   (View Abstract at PubMed)
DOI: DOI:10.1016/j.acthis.2009.08.003   (Journal Full-text)

We have used semi-quantitative RT-PCR, Western blot, and immunofluorescence imaging approaches to detect the expression levels of Slit2 and its receptor Robo1 in the rat spinal cord after traumatic lesions. Our results revealed that both the mRNA and protein levels of Slit2 were up-regulated in the injured spinal cord. The Slit2 expression level was increased at day 7 until day 14, and then returned to normal level at day 21 after injury. A double-immunolabelling study showed that Slit2 and neurofilament (NF) proteins were both localized in neurons of spinal corda cinerea. Slit2 immunopositivity was detected in neuronal plasma membranes but not in the axonal fibers. In contrast, the immunolabelling of Robo1 in the normal spinal cord was at a low level, mostly in the neurons of spinal corda cinerea, and remained unchanged at all time points following spinal cord injury (SCI). The regulation levels of Slit2 and Robo1 after traumatic lesions in the rat spinal cord are different. Our results indicate that Slit2-Robo1 might not be involved in the inhibitory environment after SCI.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Spinal Cord Injuries  ISOSlit2 (Rattus norvegicus)2316127; 2316127mRNA and protein:increased expression:spinal cordRGD 
Spinal Cord Injuries  IEP 2316127mRNA and protein:increased expression:spinal cordRGD 

Objects Annotated

Genes (Rattus norvegicus)
Slit2  (slit guidance ligand 2)

Genes (Mus musculus)
Slit2  (slit guidance ligand 2)

Genes (Homo sapiens)
SLIT2  (slit guidance ligand 2)


Additional Information