RGD Reference Report - Dysregulation of CREB binding protein triggers thrombin-induced proliferation of vascular smooth muscle cells. - Rat Genome Database

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Dysregulation of CREB binding protein triggers thrombin-induced proliferation of vascular smooth muscle cells.

Authors: Chen, J  Jiang, H  Xu, L  Zhu, LH  Wang, L  Wen, HZ  Hu, XR 
Citation: Chen J, etal., Mol Cell Biochem. 2008 Aug;315(1-2):123-30. Epub 2008 May 23.
RGD ID: 2316124
Pubmed: PMID:18496732   (View Abstract at PubMed)
DOI: DOI:10.1007/s11010-008-9795-4   (Journal Full-text)

Thrombin is a potent mitogen for vascular smooth muscle cells (VSMCs). CBP has been regarded as a potential therapeutic target on the basis of its ability to affect cell growth. Therefore we hypothesized that CBP mediates thrombin-induced proliferation of VSMCs. We constructed recombinant adenoviral vector that expresses four short hairpin RNA (shRNA) targeting rat CBP mRNA (CBP-shRNA/Ad). VSMCs were infected with CBP-shRNA/Ad and treated with thrombin. CBP level were analyzed by quantitative real-time PCR and Western blot. To evaluate VSMC proliferation, the cell cycle and DNA synthesis were analyzed by flow cytometry and (3)H-thymidine incorporation, respectively. CBP-shRNA/Ad infection inhibited thrombin-induced CBP expression in a dose-dependent manner concomitant with a decrease in the percentage of cells in the S phase and in DNA synthesis. These findings suggest that CBP plays a pivotal role in the S phase progression of VSMCs.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of G1/S transition of mitotic cell cycle  IMP 2316124 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Crebbp  (CREB binding protein)

Objects referenced in this article
Gene Smyd2 SET and MYND domain containing 2 Rattus norvegicus

Additional Information