RGD Reference Report - Glutamine-dependent asparagine synthetase in fetal, adult and neoplastic rat tissues. - Rat Genome Database

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Glutamine-dependent asparagine synthetase in fetal, adult and neoplastic rat tissues.

Authors: Huang, YZ  Knox, EW 
Citation: Huang YZ and Knox EW, Enzyme. 1975;19(5-6):314-28.
RGD ID: 2316008
Pubmed: PMID:237754   (View Abstract at PubMed)

Three enzyme reactions related to asparagine synthesis were studied in rat tissues: formation of aspartylhydroxamate, either from aspartate or by transfer from asparagine, and actual synthesis of asparagine from aspartate. Actual asparagine synthesis occurred at one-thousandth the rate of the other two reactions. Optimal conditions for quantitative assay of asparagine synthesis were determined in fetal liver extract, which is a rich source of the enzyme. Demonstrable activity in liver fell 6 days after birth to 20% of the fetal value and decreased slowly thereafter to the low adult value. Adult pancreas was the most active tissue found. The asparagine synthetase of fetal liver extracts was significantly inhibited when combined with adult liver or tumor extracts. The inhibitor fractionated with ammonium sulfate in close association with the asparagine synthetase. Therefore, demonstrable activities of asparagine synthetase in tissue extracts, measured in the presence of this inhibitor, do not necessarily parallel the concentrations of the enzyme present.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
liver development  IEP 2316008 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Asns  (asparagine synthetase (glutamine-hydrolyzing))


Additional Information