RGD Reference Report - Expression of homeobox genes in cervical cancer. - Rat Genome Database

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Expression of homeobox genes in cervical cancer.

Authors: Li, H  Huang, CJ  Choo, KB 
Citation: Li H, etal., Gynecol Oncol. 2002 Feb;84(2):216-21.
RGD ID: 2315697
Pubmed: PMID:11812077   (View Abstract at PubMed)
DOI: DOI:10.1006/gyno.2001.6498   (Journal Full-text)

OBJECTIVE: Members of the homeobox (HB) gene superfamily encode transcription factors crucial for development and may be associated with tumorigenesis. In this study, we aimed to develop a procedure to survey the expression of the dispersed-type HB genes in cervical cancer cells. METHODS: Nineteen sets of degenerate primers were designed based on conserved homeodomains of known dispersed-type HB genes. A cDNA library derived from HeLa, a cervical cancer cell line, was used. Two successive rounds of PCR were performed using a combination of the HB degenerate primers and a primer recognizing the flanking sequence of the vector used in the cDNA library construction. RESULTS: On cloning and sequence analysis of the PCR fragments generated, 10 known and 3 putative novel HB genes were detected in HeLa. RT-PCR expression analysis further showed that HOXD9 and ATBF1 were differentially expressed in cancer cells and not in normal cervix. CONCLUSIONS: Our data demonstrate the feasibility of using degenerate primers in PCR experiments in a collective analysis of complex gene families. Our data indicate that HOXD9 and ATBF1 are expressed in cervical cancer, but not in normal cervix.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cervical cancer  IEP 2315697mRNA:increased expression:uterine cervixRGD 
cervical cancer  ISOZFHX3 (Homo sapiens)2315697; 2315697mRNA:increased expression:uterine cervixRGD 

Objects Annotated

Genes (Rattus norvegicus)
Zfhx3  (zinc finger homeobox 3)

Genes (Mus musculus)
Zfhx3  (zinc finger homeobox 3)

Genes (Homo sapiens)
ZFHX3  (zinc finger homeobox 3)


Additional Information