RGD Reference Report - Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase IIalpha and active caspase-3. - Rat Genome Database

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Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase IIalpha and active caspase-3.

Authors: Coelho, RA  Focchi, GR  Nogueira-de-Souza, NC  Sartori, MG  Silva, ID  Ribalta, JC 
Citation: Coelho RA, etal., Eur J Gynaecol Oncol. 2008;29(5):499-501.
RGD ID: 2315126
Pubmed: PMID:19051821   (View Abstract at PubMed)

PURPOSE: To study the relationship between topoisomerase IIalpha, active caspase-3 expressions and HPV DNA in uterine cervices with low-grade squamous intraepithelial lesions (LSIL). METHODS: Forty women with LSIL and 32 without cervical neoplasia diagnosed through cytologic and histopathologic examination were evaluated regarding topoisomerase IIalpha and active caspase-3 expressions and HPV DNA detection using PCR (GP5/GP6) in cervicovaginal smears. RESULTS: The mean percentage of cells immunomarked by topoisomerase in the group with LSIL was 11.62% while in the control it was 4.13% (p < 0.0001). In the presence of HPV DNA, topoisomerase expression was higher in the group with productive viral infection than in nonneoplastic tissue (p = 0.004). Caspase-3 expression was observed in 17 patients with LSIL (42.5%) and in five without cervical neoplasia (15.63%). CONCLUSION: The use of topoisomerase IIalpha and active caspase-3 in cervical biopsies may help to define the prognosis of HPV cervical infection.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cervix uteri carcinoma in situ  IEP 2315126 RGD 
cervix uteri carcinoma in situ  ISOTOP2A (Homo sapiens)2315126; 2315126 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Top2a  (DNA topoisomerase II alpha)

Genes (Mus musculus)
Top2a  (topoisomerase (DNA) II alpha)

Genes (Homo sapiens)
TOP2A  (DNA topoisomerase II alpha)


Additional Information