RGD Reference Report - Increased H(2)O (2) level in exhaled breath condensate in primary breast cancer patients. - Rat Genome Database

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Increased H(2)O (2) level in exhaled breath condensate in primary breast cancer patients.

Authors: Stolarek, RA  Potargowicz, E  Seklewska, E  Jakubik, J  Lewandowski, M  Jeziorski, A  Nowak, D 
Citation: Stolarek RA, etal., J Cancer Res Clin Oncol. 2009 Dec 5.
RGD ID: 2315111
Pubmed: PMID:19967414   (View Abstract at PubMed)
DOI: DOI:10.1007/s00432-009-0734-x   (Journal Full-text)

PURPOSE: This study was designed to assess exhaled hydrogen peroxide (H(2)O(2)), blood serum antioxidant capacity, and tumor necrosis factor-alpha (TNFalpha) in primary breast cancer (PBC). METHODS: The study included 34 consecutive, non-smoking PBC patients (aged 62.5 +/- 13.5 at surgery) prior to the treatments, qualified for modified radical mastectomy and not undergoing any adjuvant systemic therapy, and 33 healthy controls. The post surgery pathological assessment included tissue expression of estrogen (ER) and progesterone (PR) receptors, and epidermal growth factor receptor type 2 (HER-2/neu). Exhaled H(2)O(2) was determined fluorometrically in the exhaled breath condensate (EBC). Blood serum antioxidant capacity and TNFalpha levels were assessed with ferric reducing ability of plasma (FRAP) and ELISA immunoassay, respectively. RESULTS: In PBC patients, 10 ER, 11 PR, and 9 HER-2/neu positive tumors were identified and HER-2/neu score was 2+ in 20% of all tumors. Median (Me) H(2)O(2) was increased up to 0.44 muM (interquartile range IR: 0.20-1.25 muM) compared with healthy control of 0.36 muM (IR: 0.12-0.48 muM; p < 0.05). The H(2)O(2) concentration in EBC was significantly correlated (tau = 0.27; p = 0.03) and increased in cases with nodal metastases (n = 12; p = 0.04). Serum TNFalpha was increased up to 51.7 +/- 21.0 pg/ml compared with controls 17.2 +/- 3.65 pg/ml (p < 0.05). FRAP was increased to 1.41 +/- 0.37 mM Fe(2+) compared with control 1.19 +/- 0.17 mM Fe(2+); (p = 0.006). CONCLUSIONS: This is the first study to demonstrate increased H(2)O(2) in exhaled breath condensate in patients with localized breast malignancy and its relation with clinical severity.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
breast cancer  IEP 2315111protein:increased expression:serumRGD 
breast cancer  ISOTNF (Homo sapiens)2315111; 2315111protein:increased expression:serumRGD 

Objects Annotated

Genes (Rattus norvegicus)
Tnf  (tumor necrosis factor)

Genes (Mus musculus)
Tnf  (tumor necrosis factor)

Genes (Homo sapiens)
TNF  (tumor necrosis factor)


Additional Information