RGD Reference Report - Microsatellite alterations in exfoliated cervical epithelia deoxyribonucleic acid as a marker for high-grade dysplasia. - Rat Genome Database

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Microsatellite alterations in exfoliated cervical epithelia deoxyribonucleic acid as a marker for high-grade dysplasia.

Authors: Chang, CL  Wang, SY  Wu, CC  Su, TH  Wang, KL  Chen, HS  Yang, YC 
Citation: Chang CL, etal., Am J Obstet Gynecol. 2001 Jul;185(1):108-15.
RGD ID: 2315099
Pubmed: PMID:11483913   (View Abstract at PubMed)
DOI: DOI:10.1067/mob.2001.114919   (Journal Full-text)

OBJECTIVE: The purpose of this study was to evaluate the feasibility of the use of deoxyribonucleic acid microsatellite alterations in cervical epithelia in the prediction of high-grade dysplasia and to compare it with a strategy based on human papillomavirus testing. STUDY DESIGN: Our prospective study subjects were from a cohort of 498 women with minimally abnormal Papanicolaou test results including atypical squamous cells of undetermined significance and low-grade squamous intraepithelial lesion who had documented repeated Papanicolaou and human papillomavirus tests. Of these, 52 eligible patients having conizations or hysterectomies as their histologic outcomes were subjected to tests of loss of heterozygosity on a panel of 5 microsatellites (D3S1110, THRB, D3S1228, D6S291, D3S1289) within the deoxyribonucleic acid of exfoliated cervical epithelia. These genetic alterations were analyzed through fluorescence polymerase chain reaction by comparison of allele ratios of exfoliated cells with those of normal control tissue. Predictive values for high-grade cervical dysplasia and cancer between this deoxyribonucleic acid marker and human papillomavirus status were compared. RESULTS: With the use of loss of heterozygosity in at least one locus for predicting high-grade cervical neoplastic lesion, the sensitivity, specificity, positive predictive value, and negative predictive value were 96.7%, 59.1%, 76.3%, and 92.9%, which were superior to those of the human papillomavirus test (80%, 59.1%, 72.7%, and 92.9%). As a triage for atypical squamous cells of undetermined significance, its sensitivity and negative predictive value were up to 100%. CONCLUSION: The promising results on determining microsatellite alteration in dysplastic lesions might imply that it is possible to detect the earliest changes by potential molecular markers with exfoliated cervical epithelial cells.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cervix uteri carcinoma in situ  IAGP 2315099DNA:loss of heterozygosity (human)RGD 
cervix uteri carcinoma in situ  ISOTHRB (Homo sapiens)2315099; 2315099DNA:loss of heterozygosity (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Thrb  (thyroid hormone receptor beta)

Genes (Mus musculus)
Thrb  (thyroid hormone receptor beta)

Genes (Homo sapiens)
THRB  (thyroid hormone receptor beta)


Additional Information