RGD Reference Report - Regulation of myosin-VI targeting to endocytic compartments. - Rat Genome Database

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Regulation of myosin-VI targeting to endocytic compartments.

Authors: Dance, AL  Miller, M  Seragaki, S  Aryal, P  White, B  Aschenbrenner, L  Hasson, T 
Citation: Dance AL, etal., Traffic. 2004 Oct;5(10):798-813.
RGD ID: 2314965
Pubmed: PMID:15355515   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1600-0854.2004.00224.x   (Journal Full-text)

Myosin-VI has been implicated in endocytic trafficking at both the clathrin-coated and uncoated vesicle stages. The identification of alternative splice forms led to the suggestion that splicing defines the vesicle type to which myosin-VI is recruited. In contrast to this hypothesis, we find that in all cell types examined, myosin-VI is associated with uncoated endocytic vesicles, regardless of splice form. GIPC, a PDZ-domain containing adapter protein, co-assembles with myosin-VI on these vesicles. Myosin-VI is only recruited to clathrin-coated vesicles in cells that express high levels of Dab2, a clathrin-binding adapter protein. Overexpression of Dab2 is sufficient to reroute myosin-VI to clathrin-coated pits in cells where myosin-VI is normally associated with uncoated vesicles. In normal rat kidney (NRK) cells, which express high endogenous levels of Dab2, splicing of the globular tail domain further modulates targeting of ectopically expressed myosin-VI. Although myosin-VI can be recruited to clathrin-coated pits, we find no requirement for myosin-VI motor activity in endocytosis in NRK cells. Instead, our data suggest that myosin-VI recruitment to clathrin-coated pits may be an early step in the recruitment of GIPC to the vesicle surface.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
endocytic vesicle  IDA 2314965 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Myo6  (myosin VI)


Additional Information