RGD Reference Report - Identification of a novel protein complex containing annexin A4, rabphilin and synaptotagmin. - Rat Genome Database
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Identification of a novel protein complex containing annexin A4, rabphilin and synaptotagmin.

Authors: Willshaw, A  Grant, K  Yan, J  Rockliffe, N  Ambavarapu, S  Burdyga, G  Varro, A  Fukuoka, S  Gawler, D 
Citation: Willshaw A, etal., FEBS Lett. 2004 Feb 13;559(1-3):13-21.
RGD ID: 2314877
Pubmed: (View Article at PubMed) PMID:14960300
DOI: Full-text: DOI:10.1016/S0014-5793(03)01513-8

Rabphilin is a synaptic vesicle-associated protein proposed to play a role in regulating neurotransmitter release. Here we report the isolation and identification of a novel protein complex containing rabphilin, annexin A4 and synaptotagmin 1. We show that the rabphilin C2B domain interacts directly with the N-terminus of annexin A4 and mediates the co-complexing of these two proteins in PC12 cells. Analyzing the cellular localisation of these co-complexing proteins we find that annexin A4 is located on synaptic membranes and co-localises with rabphilin at the plasma membrane in PC12 cells. Given that rabphilin and synaptotagmin are synaptic vesicle proteins involved in neurotransmitter release, the identification of this complex suggests that annexin A4 may play a role in synaptic exocytosis.

Annotation

Gene Ontology Annotations    

Cellular Component

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Rph3a  (rabphilin 3A)
Syt1  (synaptotagmin 1)


Additional Information