RGD Reference Report - Structural determinants for Ca2+ and phosphatidylinositol 4,5-bisphosphate binding by the C2A domain of rabphilin-3A. - Rat Genome Database

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Structural determinants for Ca2+ and phosphatidylinositol 4,5-bisphosphate binding by the C2A domain of rabphilin-3A.

Authors: Coudevylle, N  Montaville, P  Leonov, A  Zweckstetter, M  Becker, S 
Citation: Coudevylle N, etal., J Biol Chem. 2008 Dec 19;283(51):35918-28. Epub 2008 Oct 21.
RGD ID: 2314874
Pubmed: PMID:18945677   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M804094200   (Journal Full-text)

Rabphilin-3A is a neuronal C2 domain tandem containing protein involved in vesicle trafficking. Both its C2 domains (C2A and C2B) are able to bind phosphatidylinositol 4,5-bisphosphate, a key player in the neurotransmitter release process. The rabphilin-3A C2A domain has previously been shown to bind inositol-1,4,5-trisphosphate (IP3; phosphatidylinositol 4,5-bisphosphate headgroup) in a Ca2+-dependent manner with a relatively high affinity (50 microm) in the presence of saturating concentrations of Ca2+. Moreover, IP3 and Ca2+ binding to the C2A domain mutually enhance each other. Here we present the Ca2+-bound solution structure of the C2A domain. Structural comparison with the previously published Ca2+-free crystal structure revealed that Ca2+ binding induces a conformational change of Ca2+ binding loop 3 (CBL3). Our IP3 binding studies as well as our IP3-C2A docking model show the active involvement of CBL3 in IP3 binding, suggesting that the conformational change on CBL3 upon Ca2+ binding enables the interaction with IP3 and vice versa, in line with a target-activated messenger affinity mechanism. Our data provide detailed structural insight into the functional properties of the rabphilin-3A C2A domain and reveal for the first time the structural determinants of a target-activated messenger affinity mechanism.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
calcium ion binding  IDA 2314874 RGD 
inositol 1,4,5 trisphosphate binding  IDA 2314874 RGD 
phosphatidylinositol-4,5-bisphosphate binding  IDA 2314874 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Rph3a  (rabphilin 3A)


Additional Information