RGD Reference Report - Transcriptional activation of H- and N-ras oncogenes in human cervical cancer. - Rat Genome Database

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Transcriptional activation of H- and N-ras oncogenes in human cervical cancer.

Authors: Mammas, IN  Zafiropoulos, A  Koumantakis, E  Sifakis, S  Spandidos, DA 
Citation: Mammas IN, etal., Gynecol Oncol. 2004 Mar;92(3):941-8.
RGD ID: 2314838
Pubmed: PMID:14984964   (View Abstract at PubMed)
DOI: DOI:10.1016/j.ygyno.2003.11.040   (Journal Full-text)

OBJECTIVE: Overexpression of p21 protein has been detected in human cervical cancer. However, to date, there are no data on the differential activation of the three ras genes at the transcriptional level in cervical lesions. The purpose of this study was to evaluate the quantitative and qualitative changes of expression of the ras family genes in the development of human cervical cancer. METHODS: The expression of ras mRNA levels in 35 human cervical specimens [11 normal cervix, 15 cervical intraepithelial neoplasia (CIN), 9 cervical cancer] was examined using the RT-PCR technique. In addition, we studied the incidence of point mutations in codon 12 of each ras gene using RFLP analysis and human papilloma virus (HPV) status. RESULTS: The transcript levels for H-ras and N-ras were significantly higher in cancer cases compared to normal cervical tissues (P=0.0002 and P=0.001, respectively) and CIN lesions (P<0.0001 and P=0.002, respectively). The transcript levels for K-ras were similar in normal cervical tissue, CIN and cervical cancer. A strong positive correlation was found between H- and N-ras expression (P=0.001) and no correlation between H- and K- or N- and K-ras expression. Point mutations were detected only in three samples, located in codon 12 of K-ras gene. No relationship was found between expression levels of each ras gene and the presence of HPV. CONCLUSION: Our findings indicate the expression pattern of the three ras genes in cervical tissue and the involvement of H- and N-ras up-regulation in the pathogenesis of cervical cancer independent of HPV infection.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cervical cancer  IEP 2314838mRNA:increased expression:uterine cervixRGD 
cervical cancer  ISONRAS (Homo sapiens)2314838; 2314838mRNA:increased expression:uterine cervixRGD 
Uterine Cervical Neoplasms  IEP 2314838mRNA:increased expression:uterine cervixRGD 
Uterine Cervical Neoplasms  ISOHRAS (Homo sapiens)2314838; 2314838mRNA:increased expression:uterine cervixRGD 
Uterine Cervical Neoplasms  IAGP 2314838DNA:point mutation:exon (human)RGD 
Uterine Cervical Neoplasms  ISOKRAS (Homo sapiens)2314838; 2314838DNA:point mutation:exon (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Hras  (HRas proto-oncogene, GTPase)
Kras  (KRAS proto-oncogene, GTPase)
Nras  (NRAS proto-oncogene, GTPase)

Genes (Mus musculus)
Hras  (Harvey rat sarcoma virus oncogene)
Kras  (Kirsten rat sarcoma viral oncogene homolog)
Nras  (neuroblastoma ras oncogene)

Genes (Homo sapiens)
HRAS  (HRas proto-oncogene, GTPase)
KRAS  (KRAS proto-oncogene, GTPase)
NRAS  (NRAS proto-oncogene, GTPase)


Additional Information