RGD Reference Report - Reactive oxygen species are necessary for high flow (shear stress)-induced diameter enlargement of rat resistance arteries. - Rat Genome Database

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Reactive oxygen species are necessary for high flow (shear stress)-induced diameter enlargement of rat resistance arteries.

Authors: Belin de Chantemele, EJ  Vessieres, E  Dumont, O  Guihot, AL  Toutain, B  Loufrani, L  Henrion, D 
Citation: Belin de Chantemele EJ, etal., Microcirculation. 2009 Jul;16(5):391-402. Epub 2009 Apr 7.
RGD ID: 2314427
Pubmed: PMID:19353375   (View Abstract at PubMed)
DOI: DOI:10.1080/10739680902816301   (Journal Full-text)

OBJECTIVES: Chronic increases in blood flow induce remodeling associated with increases in diameter and endothelium-mediated dilation. Remodeling requires cell growth and migration, which may involve reactive oxygen species (ROS). Nevertheless, the role of ROS in flow-mediated remodeling in resistance arteries is not known. MATERIALS AND METHODS: Rat mesenteric resistance arteries (MRAs) were exposed to high flow (HF) by sequentially ligating second-order MRAs in vivo. After three weeks, arteries were collected for structural, pharmacological, and biochemical analysis. RESULTS: In HF arteries, luminal diameter (431+/-12 to 553+/-14 microm; n=10), endothelium (acetylcholine)-mediated vasodilatation (61+/-6 to 77+/-6% relaxation) and NAD(P)H subunit (gp91phox and p67phox) expression levels, and ROS (dihydroethydine microphotography) and peroxynitrite (3-nitro-tyrosine) production were higher than in normal flow arteries. Acute ROS scavenging with tempol improved acetylcholine-dependent relaxation (92+/-4% relaxation), confirming that ROS are produced in HF arteries. Chronic treatment with tempol prevented the increase in diameter, reduced ROS and peroxynitrite production, and improved endothelium-mediated relaxation in HF arteries. Thus, ROS and NO were involved in HF-induced diameter enlargement, possibly through the formation of peroxynitrite, while ROS reduced the increase in endothelium-dependent relaxation. CONCLUSIONS: ROS production is necessary for flow-mediated diameter enlargement of resistance arteries. However, ROS counteract, in part, the associated improvement in endothelium-mediated relaxation.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to laminar fluid shear stress  IEP 2314427 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ncf2  (neutrophil cytosolic factor 2)


Additional Information