RGD Reference Report - Postsynaptic shank antagonizes dendrite branching induced by the leucine-rich repeat protein Densin-180. - Rat Genome Database

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Postsynaptic shank antagonizes dendrite branching induced by the leucine-rich repeat protein Densin-180.

Authors: Quitsch, A  Berhorster, K  Liew, CW  Richter, D  Kreienkamp, HJ 
Citation: Quitsch A, etal., J Neurosci. 2005 Jan 12;25(2):479-87.
RGD ID: 2314408
Pubmed: PMID:15647492   (View Abstract at PubMed)
PMCID: PMC6725485   (View Article at PubMed Central)
DOI: DOI:10.1523/JNEUROSCI.2699-04.2005   (Journal Full-text)

Leucine-rich repeat and PDZ [postsynaptic density-95 (PSD-95)/Discs large/zona occludens-1] domain proteins such as scribble and Densin-180 have been implicated in the establishment of cell-cell contacts. Here, we show that Densin-180, which has been identified as a constituent of the postsynaptic density in excitatory synapses interacts with the postsynaptic scaffold protein shank (shank1-3). The interaction involves a two-point attachment of the C-terminal region of Densin-180 with the Src homology 3 domain and the N-terminal part of the proline-rich region of shank proteins. The N-terminal leucine-rich repeat region, which is not involved in binding shank, targets Densin-180 to the plasma membrane in transfected cells and to the basolateral membrane of epithelial cells. Nevertheless, coexpression of shank leads to a redirection of Densin-180 into intracellular clusters. In cultured hippocampal neurons, Densin-180 overexpression induces excessive branching of neuronal dendrites, which occurs at the expense of clusters for the postsynaptic marker PSD-95. Coexpression of shank3 abrogates branch formation and targets Densin-180 into postsynaptic clusters instead. Shank blocks binding of delta-catenin but not alphaCaM kinase II to Densin-180; because delta-catenin has been shown to induce branching and neurite formation, our data suggest a mechanism where shank could block the activation of a Densin-180-dependent signaling pathway by delta-catenin.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of neuron projection development  IDA 2314408 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Lrrc7  (leucine rich repeat containing 7)


Additional Information