Netrin-1 and slit-2 regulate and direct neurite growth of ventral midbrain dopaminergic neurons.

Authors: Lin, L  Rao, Y  Isacson, O 
Citation: Lin L, etal., Mol Cell Neurosci. 2005 Mar;28(3):547-55.
Pubmed: (View Article at PubMed) PMID:15737744
DOI: Full-text: DOI:10.1016/j.mcn.2004.11.009

We investigated the roles of netrin-1 and slit-2 in regulation and navigation of dopamine (DA) axon growth using an explant culture preparation of embryonic ventral midbrain (embryonic day 14) and a co-culture system. We found that netrin-1 protein significantly enhanced DA axonal outgrowth and promoted DA axonal outgrowth in a co-culture system of netrin-1 expressing cells. Such effects were mediated by the receptor DCC as demonstrated by antibody perturbation of the DCC receptor. In contrast, slit-2 inhibited DA neuron extensions and repelled DA neurite growth. These slit-2 activities required robo receptors since the reduced neurite extension was abolished by addition of excess robo receptors. In this system, netrin-1 stimulated and slit-2 opposed DA neurite growth. Such regulation may be important for DA axonal maintenance, regeneration, and phenotypic target recognition.


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RGD ID: 2314389
Created: 2009-11-12
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Last Modified: 2009-11-12
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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.