RGD Reference Report - Quantitative chemical composition of cortical GABAergic neurons revealed in transgenic venus-expressing rats. - Rat Genome Database

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Quantitative chemical composition of cortical GABAergic neurons revealed in transgenic venus-expressing rats.

Authors: Uematsu, M  Hirai, Y  Karube, F  Ebihara, S  Kato, M  Abe, K  Obata, K  Yoshida, S  Hirabayashi, M  Yanagawa, Y  Kawaguchi, Y 
Citation: Uematsu M, etal., Cereb Cortex. 2008 Feb;18(2):315-30. Epub 2007 May 20.
RGD ID: 2314348
Pubmed: (View Article at PubMed) PMID:17517679
DOI: Full-text: DOI:10.1093/cercor/bhm056

Although neocortical GABAergic (gamma-aminobutyric acidergic) interneurons have been the focus of intense study, especially in the rat, a consensus view of the functional diversity and organization of inhibitory cortical neurons has not yet been achieved. To better analyze GABAergic neurons in the rat, we used a bacterial artificial chromosome (BAC) construct and established 2 lines of transgenic rats that coexpress Venus, a yellow fluorescent protein, with the vesicular GABA transporter. The brain GABA content from both transgenic lines was similar to the level found in wild-type rats. In the frontal cortex, Venus was expressed in >95% of GABAergic neurons, most of which also expressed at least one of 6 biochemical markers, including alpha-actitin-2, which preferentially labeled late-spiking neurogliaform cells. Taking advantage of the fact that Venus expression allows for targeted recording from all classes of nonpyramidal cells, irrespective of their somatic morphologies, we demonstrated that fast-spiking neurons, which were heterogeneous in somatic size as well as vertical dendritic projection, had relatively uniform horizontal dimensions, suggesting a cell type-specific columnar input territory. Our data demonstrate the benefits of VGAT-Venus rats for investigating GABAergic circuits, as well as the feasibility of using BAC technology in rats to label subsets of specific, genetically defined neurons.


Objects referenced in this article
Strain W-Tg(Slc32a1-YFP*)1Yyan null Rattus norvegicus
Strain W-Tg(Slc32a1-YFP*)2Yyan null Rattus norvegicus

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