RGD Reference Report - The nuclear membrane organization of leukotriene synthesis. - Rat Genome Database

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The nuclear membrane organization of leukotriene synthesis.

Authors: Mandal, AK  Jones, PB  Bair, AM  Christmas, P  Miller, D  Yamin, TT  Wisniewski, D  Menke, J  Evans, JF  Hyman, BT  Bacskai, B  Chen, M  Lee, DM  Nikolic, B  Soberman, RJ 
Citation: Mandal AK, etal., Proc Natl Acad Sci U S A. 2008 Dec 23;105(51):20434-9. Epub 2008 Dec 15.
RGD ID: 2313889
Pubmed: PMID:19075240   (View Abstract at PubMed)
PMCID: PMC2629249   (View Article at PubMed Central)
DOI: DOI:10.1073/pnas.0808211106   (Journal Full-text)

Leukotrienes (LTs) are signaling molecules derived from arachidonic acid that initiate and amplify innate and adaptive immunity. In turn, how their synthesis is organized on the nuclear envelope of myeloid cells in response to extracellular signals is not understood. We define the supramolecular architecture of LT synthesis by identifying the activation-dependent assembly of novel multiprotein complexes on the outer and inner nuclear membranes of mast cells. These complexes are centered on the integral membrane protein 5-Lipoxygenase-Activating Protein, which we identify as a scaffold protein for 5-Lipoxygenase, the initial enzyme of LT synthesis. We also identify these complexes in mouse neutrophils isolated from inflamed joints. Our studies reveal the macromolecular organization of LT synthesis.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Alox5apRatleukotriene biosynthetic process  IDA  RGD 

Cellular Component

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Alox5apRatnuclear envelope  IDA  RGD 

Molecular Function

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Alox5apRatenzyme binding  IPIAlox5 (Rattus norvegicus) RGD 
Alox5Ratprotein binding  IPIAlox5ap (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Alox5  (arachidonate 5-lipoxygenase)
Alox5ap  (arachidonate 5-lipoxygenase activating protein)

Additional Information